METTL16 gene and application thereof

A gene and pancreatic cancer technology, applied in the field of tumor molecular biology, can solve the problems of low sensitivity and specificity, overtreatment, etc., and achieve the effects of improving specificity, improving timeliness, and enhancing invasion and metastasis capabilities

Pending Publication Date: 2022-06-03
广州汇奇生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is that the existing molecular markers for the diagnosis of pancreatic cancer have the defects of low sensitivity and specificity, and are prone to overtreatment, so as to provide a pancreatic cancer-related marker with high specificity and sensitivity. Molecular markers of

Method used

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  • METTL16 gene and application thereof
  • METTL16 gene and application thereof
  • METTL16 gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] This embodiment provides a method for detecting the mRNA expression levels of 21 genes in pancreatic cancer tissue (Tumor, referred to as T) and normal tissue (Normal, referred to as N) by using bioinformatics analysis, the specific content is as follows:

[0061] Get METTL3, METTL5, METTL14, METTL16, RBM15, VIRMA, WTAP, CBLL1, IGF2BP1, IGF2BP2, IGF2BP3, HNRNPC, HNRNPA2B1, YTHDC1 from the Oncomine online database (https: / / www.oncomine.org / resource / login.html) , YTHDC2, YTHDF1, YTHDF2, YTHDF3, RBMX, FTO, ALKBH5 gene expression in pan-cancer. Download pancreas cancer (Pancreatic cancer or Pancreatic Adenocarcinoma, PAAD) gene-wide single nucleotide polymorphisms ( Single nucleotide polymorphism, SNP) and transcriptome expression data, analyzed in the case of full mutations in key driver genes (TP53, KRAS, CDKN2A and SMAD4), METTL3, METTL5, METTL14, METTL16, RBM15, VIRMA, WTAP, CBLL1, IGF2BP1, IGF2BP2, Differential expression analysis of IGF2BP3, HNRNPC, HNRNPA2B1, YTHDC1...

Embodiment 2

[0064] This embodiment provides the use of bioinformatics analysis, and this embodiment provides a method for detecting the correlation between the expression of METTL16 gene and the cancer prognosis of pancreatic cancer, the specific content is as follows:

[0065] The Kaplan-Meier Plotter (http: / / kmplot.com / analysis / ) database was used to analyze the relationship between METTL16 expression and prognosis of pancreatic cancer. The Kaplan-Meier Plotter database contains mRNA sequencing and follow-up information of 177 pancreatic ductal adenocarcinoma (PDAC or PAAD) patients. The samples are divided into high and low expression groups according to the mean of each gene expression. Survival function curve of logrank p (logrank p), the survival curve represents the correlation between METTL16 gene and pancreatic cancer (PAAD) Overall Survival (OS) and Disease Free Survival (DFS), independent The screening analysis of clinical factors was performed by univariate Cox regression anal...

Embodiment 3

[0069] This example provides a method for evaluating the diagnostic value of METTL16 gene expression level for pancreatic cancer, the specific content is: download the TPM of TCGA and GTEx in Example 2 through UCSC XENA (https: / / xenabrowser.net / datapages / ) Format RNAseq data, retained clinical data and removed duplicate samples, a total of 179 cases of TCGA PAAD and 171 cases of GTEx corresponding normal tissue data were obtained. The acquired pancreatic cancer patient data and the corresponding normal tissue data were edited by software to obtain the ROC (receiver operator characteristic curve) curve.

[0070] The ROC curve can be drawn using software or systems in the prior art, such as = MedCalc9.2.0.1 medical statistics software, SPSS 9.0, ROCP0WER.SAS, DESIGNROC.FOR, MULTIREADER_POWER.SAS, R-studio, CREATE-ROC.SAS >GB STAT V10.0 (Dynamic Microsystems, Inc. Silver Spring, MD, USA) and so on. The software used to draw the ROC curve in this example is R-studio.

[0071] Th...

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Abstract

The invention provides a pancreatic cancer marker. The pancreatic cancer marker is an METTL16 gene or an expression product of the METTL16 gene. Researches find that the expression level of the METTL16 gene in pancreatic cancer tissues is significantly lower than the expression level in normal tissues, which indicates that the expression level of the METTL16 gene has significant correlation with pancreatic cancer. The METTL16 gene and/or the expression product of the METTL16 gene are/is an ideal target spot for early molecular diagnosis and disease risk screening of pancreatic cancer, so that the timeliness of pancreatic cancer diagnosis is improved, and the specificity of pancreatic cancer diagnosis is improved. The METTL16 gene and/or the expression product of the METTL16 gene are/is used as diagnosis indexes of pancreatic cancer noninvasive screening, clinical treatment effects of pancreatic cancer are monitored, important clinical information is provided for prevention, early discovery and early treatment of pancreatic cancer, and improvement of the survival quality of patients and improvement of the survival rate of the patients are facilitated.

Description

technical field [0001] The present invention relates to the field of tumor molecular biology, in particular to METTL16 gene and use thereof. Background technique [0002] Pancreatic cancer is one of the gastrointestinal tumors with the worst clinical prognosis in my country. Early diagnosis is difficult, the 5-year survival rate is extremely low, and recurrence is easy. Pancreatic cancer has become a potential malignant disease that seriously affects people's health. Surgery remains the preferred treatment option for pancreatic cancer, but due to the difficulty of early diagnosis, only about 15% of patients undergo radical surgical resection. At the same time, chemotherapy and radiotherapy are not satisfactory for patients with advanced pancreatic cancer. The key to the diagnosis and treatment of pancreatic cancer lies in early detection, accurate diagnosis and timely intervention. Pancreatic cancer mortality can be significantly reduced by early screening control. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574C12N15/867C12N5/10C12N15/113A61K45/00A61P35/00A61P35/04
CPCC12Q1/6886G01N33/57423G01N33/57484C07K14/47C12N15/86C12N15/1135A61K45/00A61P35/00A61P35/04C12Q2600/106C12Q2600/158C12Q2600/118C12Q2600/136G01N2800/52C12N2310/14C12N2740/15043
Inventor 黄凤婷张世能陈文颖卢丽婷郑丹丹
Owner 广州汇奇生物技术有限公司
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