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Sanhua decoction reference sample freeze-dried powder as well as preparation method and quality detection method thereof

A quality inspection method and reference sample technology, applied in the preparation of test samples, measuring devices, instruments, etc., can solve the problems of one-by-one color development or different detection conditions, rough process, many spots, etc., to achieve effective quality control , comprehensive detection methods, and unified index components

Pending Publication Date: 2022-06-03
SHAANXI UNIV OF SCI & TECH
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  • Abstract
  • Description
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Problems solved by technology

[0004] Patent document CN108956845B discloses a multi-information thin-layer identification method of Sanhua decoction freeze-dried powder, one board and four medicinal materials, but its prescription dosage and water addition amount are wrong (it needs to be equivalent to the ancient dynasty, not modern), and the medicinal materials have not been processed, that is, the medicinal materials Incorrect use, no numerical value in the preparation method, too rough process, difficult to verify the authenticity of data and pictures
Although it uses multiple tests on one panel, the color of the medicines still needs to be developed one by one or the detection conditions are different. As shown in the attached picture, there are too many spots due to the simultaneous detection of multiple medicines, which is not intuitive to observe, but it is easy to cause interference.

Method used

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  • Sanhua decoction reference sample freeze-dried powder as well as preparation method and quality detection method thereof
  • Sanhua decoction reference sample freeze-dried powder as well as preparation method and quality detection method thereof
  • Sanhua decoction reference sample freeze-dried powder as well as preparation method and quality detection method thereof

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Experimental program
Comparison scheme
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Embodiment 1

[0107] Embodiment 1 A kind of optimization of the preparation method of Sanhua Tang benchmark sample freeze-dried powder, comprising the following steps:

[0108] S1. Prescription dose investigation

[0109] The present invention compares three different water addition amounts of 2100ml, 2300ml and 2500ml, and finds that with the increase of water amount, such as Figure 5 , the creaming rate also increases, but the overall difference is not large, and in terms of peak output, when 2100ml of water is added, it is obviously better; so 2100ml is selected as the total amount of water.

[0110] S2. Investigation on particle size of decoction pieces

[0111] In the present invention, two kinds of sieves, 10-mesh and 5-mesh, are used to investigate the particle size of the decoction pieces: soybean size and mung bean size. image 3 and 4 , the result is that both the paste yield and the peak yield are the same. In order to reduce the loss, the size of soybean is selected for deco...

Embodiment 2

[0125] Embodiment 2 A series of methods for controlling the quality of the lyophilized powder of the Sanhua Tang benchmark sample by thin-layer chromatography, comprising the following steps:

[0126] S1. Optimization of the detection method of anthraquinones in rhubarb

[0127] (1) Optimization of the preparation of the test product

[0128] The present invention compares the different sampling amounts (1.25g, 5.0g) of the freeze-dried powder of the Sanhua Tang benchmark sample and whether it is evaporated to dryness after heating and refluxing, such as Figure 8 , it was found that taking 5.0g of the lyophilized powder of the Sanhua Tang benchmark sample, and not steaming to dryness after heating and refluxing, the spotting effect on the thin-layer board was good, so this method was used to prepare the test sample.

[0129] (2) Optimization of the spot amount of the standard solution

[0130] In the present invention, the experimental comparison is carried out on different...

Embodiment 3

[0169] Embodiment 3 adopts high performance liquid chromatography to measure the magnolol and the total content of magnolol in the freeze-dried powder Magnolia officinalis of the Sanhua Tang benchmark sample, comprising the following steps:

[0170] S1. Preparation of reference substance solution: Take magnolol and magnolol reference substance, add methanol to prepare reference substance solutions with concentrations of 60.294 μg / ml and 60.176 μg / ml respectively;

[0171] S2, the preparation of the test solution: take the 2.0g Sanhua Tang benchmark sample freeze-dried powder of Example 1 and place it in a 250ml conical flask with a stopper, add 25ml of methanol, ultrasonically treat it for 45min, and filter to obtain the test solution ;

[0172] S3. Preparation of negative sample solution: take three kinds of decoction pieces except Magnolia officinalis, prepare freeze-dried powder according to the method of Example 1, take 2.0g and place it in a 250ml conical flask with stopp...

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Abstract

According to the Sanhua decoction reference sample freeze-dried powder as well as the preparation method and the quality detection method thereof, the freeze-dried powder is prepared by optimizing the preparation method of the Sanhua decoction reference sample freeze-dried powder, modern process parameters of a classical famous prescription reference sample are provided, and subsequent pilot plant test and industrial production are facilitated. The quality of Sanhua decoction reference sample freeze-dried powder is detected by two technical means of thin-layer chromatography and high-phase liquid chromatography, all medicinal materials in a formula are identified by adopting the thin-layer chromatography, an independent TLC detection means is formulated for the medicinal materials according to the difference of different medicinal material components, rhaponticin detection is added, and the method is used for anti-counterfeiting, anti-counterfeiting, anti-counterfeiting, anti-counterfeiting and anti-counterfeiting of rhubarb medicinal materials. Compared with the prior art, the method is more visual and clearer. The total content of magnolol and honokiol is determined by high performance liquid chromatography, and a quantitative monitoring means is provided.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to the preparation of Sanhua Decoction, a classic famous prescription of traditional Chinese medicine, and a set of quality control methods, in particular to a freeze-dried powder of a reference sample of Sanhua Decoction, a preparation method and a quality detection method thereof. Background technique [0002] Classical prescriptions are the pearls in the treasure house of Chinese medicine, and are effective prescriptions that have been proven over time. Through inheritance, screening, mining and innovation, the theory of classical prescriptions can be explained, and their clinical efficacy, safety and economy can be clarified. The academic value and clinical value of the classic prescriptions can be further demonstrated and improved. [0003] Sanhua Decoction was first published in "Su Wen's Pathogenesis, Qi, Qi and Life-saving Collection: Stroke Theory No. 10", and is n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N30/90G01N30/02G01N30/06
CPCG01N1/28G01N30/90G01N30/02G01N30/06G01N2030/042Y02A50/30
Inventor 梁承远张洁刘玉婷闫文菁惠楠张德柱谢晓林谢晓锋谢秋芳葛跃王文学肖军毋均娥
Owner SHAANXI UNIV OF SCI & TECH
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