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Nanometer preparation of porous Pt nanoflower loaded lactate oxidase as well as preparation and application of nanometer preparation

A lactate oxidase and nano-formulation technology is applied in the field of porous Pt nanoflower-loaded lactate oxidase-loaded nano-formulations and its preparation, so as to achieve the effects of improving the killing effect, increasing the level of ROS, and enhancing the DNA damage of the nucleus.

Pending Publication Date: 2022-06-10
FUJIAN PROVINCIAL HOSPITAL
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Problems solved by technology

In order to overcome the insufficiency of a single low-dose radiotherapy effect, a synergistic treatment strategy of combined radiotherapy was constructed, and the nano-preparation surface PEGylated to make it have long-term circulation characteristics in the body, and by enhancing the penetration and retention effects, the effect of passive tumor targeting was achieved.

Method used

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  • Nanometer preparation of porous Pt nanoflower loaded lactate oxidase as well as preparation and application of nanometer preparation
  • Nanometer preparation of porous Pt nanoflower loaded lactate oxidase as well as preparation and application of nanometer preparation
  • Nanometer preparation of porous Pt nanoflower loaded lactate oxidase as well as preparation and application of nanometer preparation

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preparation example Construction

[0045] 1. Preparation of Porous Pt Nanoflower Pt NFs

[0046] Weigh Pluronic F-127 (127mg) and dissolve it in 3.6mL mixed solution (containing 0.6mL Na 2 PdCl 4 (20mM), 1.2mL K 2 PtCl 4 (20mM), 1.8mL H 2 PtCl 6 (20mM)). 240 μL of HCl solution (6M) was added to adjust the pH of the reaction solution. Then, 30 mL of ascorbic acid (AA) solution (0.1 M) was rapidly injected into the above solution, and the reaction was carried out by ultrasound in a water bath at 40° C. for 5 h. The product was centrifuged at 15,000 rpm for 10 min, washed twice with deionized water and absolute ethanol, dispersed in deionized water, and freeze-dried to obtain platinum nanoflower powder.

[0047] Take a certain mass of platinum nanoflower powder and SH-PEG5000 (m:m=1:2) and resuspend in deionized water by ultrasonic, stir at room temperature for 48h, centrifuge the product at 15000rpm for 10min, wash it with deionized water for 3 times, and disperse it in deionized water. The PEG-modified p...

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Abstract

The invention discloses a nano preparation of porous Pt nanoflowers loaded with lactate oxidase, a preparation method of the nano preparation and application of the nano preparation in preparation of radiotherapy and chemotherapy combined sensitization anti-tumor drugs. The nano preparation takes porous Pt nanoflowers as a carrier to carry chemotherapeutic drugs, simultaneously carries lactate oxidase, and is coated with a cRDG modified liposome membrane. According to the nano preparation, drug intratumoral permeation is realized by using lactate oxidase-lactic acid substrate chemotaxis, a novel enzyme chemotaxis strategy taking lactic acid as a substrate is disclosed, and the application of a nano carrier in anti-tumor drug permeation is expanded.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparations, in particular to a nano preparation of porous Pt nanoflowers loaded with lactate oxidase and a preparation method and application thereof. Background technique [0002] Breast cancer is one of the most common malignant tumors in women worldwide, and its morbidity and mortality are increasing year by year and showing a younger trend. At present, radiotherapy (radiotherapy) is one of the clinical standard treatments for locally advanced breast cancer. About 70% of breast cancer patients need to receive radiotherapy, which is attributed to the fact that high-energy ionizing radiation with strong tissue penetration can directly ionize Deep tumor cell nuclear DNA, or through the radiolysis of water and organic matter in tissue cells to generate a variety of reactive oxygen species to enhance DNA damage, thereby effectively killing tumor cells. However, the current tumor radiotherapy researc...

Claims

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Application Information

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IPC IPC(8): A61K38/44A61K31/704A61K47/02A61K47/24A61K47/18A61P35/00
CPCA61K38/443A61K31/704A61K47/02A61K47/24A61K47/18A61P35/00A61K2300/00Y02A50/30
Inventor 吴晓丹汤奕洁柯鹏黄析蕾张振涛韩旻
Owner FUJIAN PROVINCIAL HOSPITAL
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