Composite nanoparticles with tumor targeting and radiotherapy sensitization characteristics, and preparation and application of composite nanoparticles

A composite nanoparticle and tumor-targeting technology, which is applied in the field of composite nanoparticle and its preparation and application, can solve the problems of inaccurate tumor tissue localization diagnosis, accelerate DNA radiation damage repair, reduce the effect of radiotherapy cell killing, etc., and achieve enhanced tumor The effect of local radiation dose, good water dispersibility and biocompatibility, easy implementation and promotion

Inactive Publication Date: 2019-12-06
祝守慧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hypoxia is a common feature of most solid tumors. Hypoxia can cause radiotherapy resistance of tumor cells, increase the proportion of radiotherapy-insensitive G1 / S phase cells in tumor cells, and accelerate the repair of DNA radiation damage, reducing the risk of radiotherapy. cell killing effect
In addition, the positioning diagnosis of tumor tissue is not accurate enough, resulting in the inability to effectively distinguish tumor tissue from surrounding normal tissue during tumor radiotherapy, which will also reduce the radiotherapy efficiency and increase radiation side effects

Method used

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  • Composite nanoparticles with tumor targeting and radiotherapy sensitization characteristics, and preparation and application of composite nanoparticles
  • Composite nanoparticles with tumor targeting and radiotherapy sensitization characteristics, and preparation and application of composite nanoparticles
  • Composite nanoparticles with tumor targeting and radiotherapy sensitization characteristics, and preparation and application of composite nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This embodiment provides a composite nanoparticle with tumor targeting and radiosensitization properties, wherein the composite nanoparticle includes hyaluronic acid and bismuth trioxide and ceria grown on the hyaluronic acid.

[0037] The mass ratio of hyaluronic acid, bismuth trioxide and ceria in the composite nanoparticles is 50:8:1.

[0038] The method for preparing composite nanoparticles with tumor targeting and radiosensitization properties includes the following steps:

[0039] S1, dissolving bismuth chloride in ultrapure water, vigorously stirring and dissolving to a transparent homogeneous solution, the concentration of obtaining bismuth chloride is 0.02mol / L;

[0040] S2, dissolving sodium hydroxide in ultrapure water, stirring vigorously to dissolve it to a transparent homogeneous solution, and obtaining a concentration of sodium hydroxide of 1 mol / L;

[0041] S3, adding an equal volume of 1 mol / L sodium hydroxide solution to the solution prepared in step ...

Embodiment 2

[0050] In this example, the ability of the composite nanoparticles with tumor targeting and radiosensitization properties prepared in Example 1 to catalyze the decomposition of hydrogen peroxide was measured, and the method was as follows:

[0051] To 0.1 mmol / L hydrogen peroxide solution, different concentrations of the composite nanoparticles with tumor targeting and radiosensitization properties prepared in Example 1 were respectively added, so that the final concentration of cerium in the mixed solution was 0 and 0.05 mmol / L, respectively. L and 0.1 mmol / L, the dissolved oxygen concentration in the mixed solution was measured by a dissolved oxygen analyzer (JPSJ-605F, Thunder Magnetic).

[0052] The results are as figure 2 As shown, the addition of composite nanoparticles with tumor-targeting and radiosensitizing properties resulted in a rapid rise in the dissolved oxygen concentration in the solution, with a higher rate of oxygen generation in the presence of high concen...

Embodiment 3

[0054] In this example, the magnetic resonance imaging contrast ability of the composite nanoparticles with tumor targeting and radiosensitization properties prepared in Example 1 was evaluated in response to acidic pH / hydrogen peroxide, and the method was as follows:

[0055] To pH 5.0, pH 6.5 and pH 7.4 phosphate buffer and pH 5.0, pH 6.5 and pH 7.4 phosphate buffer containing 1 mM hydrogen peroxide, respectively, were added different concentrations of the tumor-targeted tumor-targeting solution prepared in Example 1 The composite nanoparticles with radiosensitizing properties were incubated at 37°C for 4 hours and then subjected to T1-weighted magnetic resonance imaging at a field strength of 7.0T.

[0056] The result is as image 3 As shown in (a), in the absence of hydrogen peroxide, ceria decomposes under acidic conditions to release Ce 4+ , to achieve acidic pH-responsive magnetic resonance imaging contrast.

[0057] The result is as image 3 As shown in (b), in the ...

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Abstract

The invention discloses composite nanoparticles with tumor targeting and radiotherapy sensitization characteristics, and preparation and application of the composite nanoparticles. The composite nanoparticles comprise hyaluronic acid, bismuth trioxide and cerium oxide which grow on the hyaluronic acid. The synthesized composite nanoparticles with the tumor targeting and radiotherapy sensitizationcharacteristics have good water dispersibility and biocompatibility, sensitization and radiotherapy are conducted through two aspects of enhancing local radiation dose of tumor by the bismuth trioxideand improving the tumor hypoxia by the cerium oxide, and meanwhile, computed tomography, magnetic resonance and photoacoustic multi-mode imaging radiography can be achieved; the preparation method issimple, conditions are mild, controllability is good, an additional organic stabilizer / dispersant does not need to be added, and implement and promotion are easy; and the integration of tumor targeted radiotherapy and sensitization diagnosis and treatment can be achieved, and wide application prospects in the fields of nano medicine, disease diagnosis and tumor treatment and the like are achieved.

Description

technical field [0001] The invention relates to the technical field of nano-composite materials, in particular to a composite nano-particle with tumor targeting and radiotherapy sensitization properties and its preparation and application. Background technique [0002] The incidence of malignant tumors in the world is increasing year by year, and the mortality rate has remained high, which seriously threatens the survival and health of human beings. Radiation therapy, as a non-invasive tumor treatment method, is widely used in the treatment of different types of tumors. In tumor radiotherapy, after tumor cells absorb a certain amount of radiation energy, radiation can directly interact with intracellular biological macromolecules to generate free radicals, causing DNA molecules to break and cross-link; radiation can also cause ionization of water molecules in tissues to generate free radicals , these free radicals interact with biological macromolecules to cause cell damage...

Claims

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Application Information

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IPC IPC(8): A61K49/22A61K49/18A61K49/12A61K49/04A61K41/00A61P35/00
CPCA61K41/0038A61K49/0002A61K49/04A61K49/124A61K49/1863A61K49/225A61P35/00
Inventor 祝守慧左丙丽
Owner 祝守慧
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