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Kit, reaction device and detection method

A technology of a reaction device and a kit, applied in the field of biological detection, can solve the problems of low concentration of nucleic acid molecules and difficult identification.

Pending Publication Date: 2022-06-28
XIAMEN ZEESAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The concentration of nucleic acid molecules after extraction is very low, making it difficult to identify with naked eyes or instruments

Method used

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  • Kit, reaction device and detection method
  • Kit, reaction device and detection method
  • Kit, reaction device and detection method

Examples

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Embodiment Construction

[0054] Various exemplary embodiments of the present disclosure will now be described in detail with reference to the accompanying drawings. The description of the exemplary embodiments is illustrative only, and in no way restricts the disclosure, its application or uses. The present disclosure can be implemented in many different forms and is not limited to the embodiments described here. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art. It should be noted that relative arrangements of parts and steps, compositions of materials, numerical expressions and numerical values ​​set forth in these embodiments should be interpreted as illustrative only and not as limiting, unless specifically stated otherwise.

[0055] "First", "second" and similar words used in the present disclosure do not indicate any order, quantity or importance, but are only used to distin...

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Abstract

The invention relates to a kit, a reaction device and a detection method. The kit comprises: a housing (11) having an internal chamber (A); at least one temperature conducting member (12) located at least partially in the interior chamber (A) and configured to obtain energy from an external heat source (22) to form a temperature conducting region in the interior chamber (A); at least one fluid channel (13) located in the internal chamber (A) and passing through or against a surface of the at least one temperature conducting member (12), configured for the flow of a reagent fluid and for heat exchange with the at least one temperature conducting member (12); wherein the at least one fluid channel (13) is provided with at least one connector which is located on the outer wall of the shell (11) or penetrates out of the outer wall of the shell (11). According to the embodiment of the invention, the transfer and temperature of the reagent fluid can be better controlled.

Description

technical field [0001] The disclosure relates to the field of biological detection, in particular to a kit, a reaction device and a detection method. Background technique [0002] In the field of molecular diagnosis, it is usually necessary to extract nucleic acid from human samples and then perform nucleic acid detection. The concentration of nucleic acid molecules after extraction is very low, making it difficult to identify with naked eyes or instruments. In related technologies, related detection is performed after the concentration of nucleic acid molecules is significantly increased, and the process of increasing the concentration of nucleic acid molecules is called nucleic acid amplification. [0003] Common amplification methods include polymerase chain reaction (Polymerase Chain Reaction, PCR for short) amplification method, isothermal amplification method, and the like. These methods require that sample nucleic acid and amplification reagents be placed together i...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/34C12M1/02C12M1/00C12Q1/686C12Q1/6844
CPCB01L7/52B01L7/00C12Q1/686C12Q1/6844C12Q2527/101C12Q2563/107
Inventor 占伟王柳艺唐宇
Owner XIAMEN ZEESAN BIOTECH
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