Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cryopreservation liquid and cryopreservation method for stem cells and stem cell products

A cryopreservation method and stem cell technology, applied in the preservation, application, animal husbandry and other directions of human or animal bodies, can solve the problems of decreased viability, hidden dangers, unstable batches, etc., to reduce usage, lower freezing point, Long-term effective effect of cryopreservation

Pending Publication Date: 2022-07-29
宁波建顺生物科技有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

DMSO is a cryoprotectant, which can protect cells from the damage of crystal substances during the cooling process, but the higher the content, the greater the toxic effect on cells, while FBS introduces animal-derived components into human cells, which has certain safety hazards , such as the introduction of mycoplasma, bovine pathogenic bacteria, etc., and batch instability
If FBS is not added, the viability of cells will drop significantly after thawing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cryopreservation liquid and cryopreservation method for stem cells and stem cell products
  • Cryopreservation liquid and cryopreservation method for stem cells and stem cell products
  • Cryopreservation liquid and cryopreservation method for stem cells and stem cell products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A cryopreservation solution for stem cells and their products, including the following raw materials in parts by weight: 10 parts of trehalose, 10 parts of EDTA, 5 parts of methylcellulose, 3 parts of glycerol, 3 parts of dimethyl sulfoxide and MEM medium 100 copies.

[0027] The cryopreservation method for stem cells and their products, using the cryopreservation solution, comprises the following steps:

[0028] (1) Preparation of cryopreservation solution: Under aseptic conditions, take each raw material according to the above ratio, add trehalose to the medium, and after mixing, add EDTA and methylcellulose in turn, and after mixing, add glycerol and dimethyl sulfoxide, and after thorough mixing, use a filter to sterilize to obtain a cryopreservation solution;

[0029] (2) Stem cell treatment: Take out stem cells from the culture flask, add trypsin for digestion, centrifuge, discard the supernatant, add normal saline to wash, and then perform centrifugation, discard...

Embodiment 2

[0032] A freezing solution for stem cells and their products, including the following raw materials by weight: 15 parts trehalose, 20 parts EDTA, 8 parts methyl cellulose, 5 parts glycerol, 5 parts dimethyl sulfoxide and DMEM medium 120 copies.

[0033] The cryopreservation method for stem cells and their products, using the cryopreservation solution, comprises the following steps:

[0034] (1) Preparation of cryopreservation solution: Under aseptic conditions, take each raw material according to the above ratio, add trehalose to the medium, and after mixing, add EDTA and methylcellulose in turn, and after mixing, add glycerol and dimethyl sulfoxide, and after thorough mixing, use a filter to sterilize to obtain a cryopreservation solution;

[0035] (2) Stem cell treatment: Take out stem cells from the culture flask, add trypsin for digestion, centrifuge, discard the supernatant, add normal saline for washing, and then perform centrifugation. After discarding the supernatant,...

Embodiment 3

[0038] A cryopreservation solution for stem cells and their products, including the following raw materials by weight: 12 parts of trehalose, 18 parts of EDTA, 6 parts of methylcellulose, 4 parts of glycerol, 4 parts of dimethyl sulfoxide and IMEM medium 110 servings.

[0039] The cryopreservation method for stem cells and their products, using the cryopreservation solution, comprises the following steps:

[0040] (1) Preparation of cryopreservation solution: Under aseptic conditions, take each raw material according to the above ratio, add trehalose to the medium, and after mixing, add EDTA and methylcellulose in turn, and after mixing, add glycerol and dimethyl sulfoxide, and after thorough mixing, use a filter to sterilize to obtain a cryopreservation solution;

[0041] (2) Stem cell treatment: Take out stem cells from the culture flask, add trypsin for digestion, centrifuge, discard the supernatant, add normal saline for washing, and then perform centrifugation. After dis...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a cryopreservation solution and a cryopreservation method for stem cells and products thereof. The cryopreservation liquid comprises the following raw materials in parts by weight: 10-15 parts of trehalose, 10-20 parts of EDTA (Ethylene Diamine Tetraacetic Acid), 5-8 parts of methylcellulose, 3-5 parts of glycerol, 3-5 parts of dimethyl sulfoxide and 100-120 parts of a culture medium. The cryopreservation liquid is prepared from trehalose, EDTA, methyl cellulose, glycerin, dimethyl sulfoxide and a culture medium, trehalose can serve as a protection component of the cryopreservation liquid, and EDTA can provide a reduction environment for cryopreserved cells; the methylcellulose can improve the anti-apoptosis and anti-oxidation capacities of the frozen stock solution after recovery; glycerin and dimethyl sulfoxide are used in cooperation, so that permeable components of the cryopreservation liquid can rapidly penetrate through cell membranes to enter cells, the freezing point is reduced, the cryopreservation process is delayed, the ion concentration in the cells is improved, formation of ice crystals in the cells is reduced, and therefore the cell damage degree is reduced; the potential toxic effect on cells is reduced.

Description

technical field [0001] The invention relates to a cryopreservation solution and a cryopreservation method for stem cells and products thereof, belonging to the technical field of cryopreservation solutions. Background technique [0002] Mesenchymal stem cells (MSCs) are derived from mesoderm tissue and belong to pluripotent stem cells. They are characterized by a wide range of sources, low immunogenicity, certain differentiation potential, self-renewal ability, and the ability to regulate immunity. Its sources are mainly divided into human umbilical cord mesenchymal stem cells, adipose mesenchymal stem cells, bone marrow mesenchymal stem cells, dental pulp mesenchymal stem cells, placental mesenchymal stem cells, etc. [0003] At present, the cryopreservation of mesenchymal stem cells is mainly 5%-20% fetal bovine serum (FBS) + cell basal medium (Dulbecco's medium or α-MEM or F12 / DMEM) containing bovine-derived serum components. ) + 10% DMSO. DMSO is a cryoprotectant, whic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 张顺阮春燕施晓峰蔡挺张沛瑶
Owner 宁波建顺生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com