Acidic virus nucleic acid degradation and disinfection method

Abstract

The invention discloses an acidic virus nucleic acid degradation and disinfection method, which comprises the following steps: (1) preparing a liquid component of an acidic nucleic acid degradation disinfectant, the liquid component comprising the following components by weight: 1-30% of peracetic acid, 1-10% of lauryl sodium sulfate, 1-10% of citric acid, 1-10% of formic acid, and the balance of water; (2) preparing a solid component of the acidic nucleic acid degradation disinfectant, wherein the solid component is prepared from the following components in parts by weight: 10-30 parts of sodium percarbonate, 10-50 parts of potassium peroxymonosulfate, 5-15 parts of potassium chloride and 10-30 parts of sodium chloride; and (3) the liquid component and the solid component are mixed according to the mass ratio of 1: 3-3: 1, and the object to be disinfected is disinfected after the solid component is completely dissolved and diluted by 50-500 times, and the disinfection time is 1-60 min.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for degrading and sterilizing acidic viral nucleic acid. Background technique [0002] Common disinfectants often can only kill pathogens, but cannot effectively degrade nucleic acids. In the process of detecting pathogens by real-time PCR method, positive results may be detected due to the presence of nucleic acid fragments, and the live or dead pathogens cannot be distinguished, resulting in false positive results. According to reports, in the case of using conventional disinfection methods, even if a 100% killing effect on pathogens is produced, dead pathogens and nucleic acids still exist, and the nucleic acid fragments of pathogens can still be detected when using fluorescence quantitative PCR. It will lead to misjudgment of the epidemic. Especially for infectious diseases such as African swine fever, even if the farm is completely killed by disinf...

AI Technical Summary

Problems solved by technology

However, the use of ethidium monoazide as a disinfectant has the disadvantage of persistent toxicity to water bodies, animals and plants due to the high toxicity of ethidium monoazide itself.

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