Functionalized inorganic fluorescent microsphere based on nitrogen-doped carbon quantum dots and preparation method thereof

Abstract

The invention relates to a functionalized inorganic fluorescent microsphere based on nitrogen-doped carbon quantum dots and a preparation method thereof, and the preparation method comprises the following steps: carrying out heating reaction on a heating reaction product of Chinese cabbage juice, ammonia water, urea, diethylenetriamine or polyethylene polyamine to prepare nitrogen-doped carbon quantum dots NCDs, and reacting with NaCl, urea and formaldehyde to obtain a urea-formaldehyde resin fluorescent microsphere. And reacting with ethanol and tetraethoxysilane to obtain the hydroxyurea-formaldehyde resin fluorescent microspheres. The green and non-toxic Chinese cabbage carbon quantum dots are prepared by adopting a simple and feasible method, and better fluorescence performance is presented after nitrogen doping; the hydroxyl urea-formaldehyde resin fluorescent microspheres are obtained by applying the hydroxyl urea-formaldehyde resin as a fluorescent probe to preparation of the fluorescent microspheres, and the hydroxyl urea-formaldehyde resin fluorescent microspheres have the advantages of good monodispersity, rich functional group content, easy combination with biomolecules, good biocompatibility and low toxicity, and have good application prospects in the fields of biology and medicine.

Description

technical field [0001] This application is a divisional application filed against the patent application with the original application number of 201810007274.1 and the invention-creation name of a functionalized inorganic fluorescent microsphere of nitrogen-doped carbon quantum dots and its preparation method. [0002] The invention belongs to the technical field of preparation methods of functionalized inorganic fluorescent microspheres, in particular to a functionalized inorganic fluorescent microspheres based on nitrogen-doped carbon quantum dots and a preparation method thereof. Background technique [0003] Carbon quantum dots (CDs), as a new type of fluorescent probes, have broad and continuous excitation spectrum, narrow and symmetrical emission spectrum, good biocompatibility and low toxicity compared with traditional semiconductor quantum dots and organic dyes It has good application prospects in biomedicine, labeling, heavy metal detection and other fields. Howeve...

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Problems solved by technology

[0004] Melamine formaldehyde microspheres are rich in amino, imino and hydroxyl groups, which can supply a large number of adsorption active sites for the adsorption of heavy metal ions Pb 2+ , but its adsorption active sites are rarely used in biology (Colloids and Surfaces A: Physicochem. Eng. Aspects, 2015, 482, 491-499 and Chemical Engineering Journal, 2016, 288, 745-757)

There are many reports on the preparation methods of urea-formaldehyde resin microspheres, but the introduction of functional groups on the surface is neglected, which is inconvenient for subsequent biological studies (Materials Letters, 2015, 145, 27-29 and Journal of Industrial and Engineering Chemistry, 201, 218,919-925)

Both monodisperse macroporous silica microspheres and mercaptolated silica microspheres can be used for the separation of proteins, but the preparation process of macroporous silica microspheres is complicated, and mercaptolated silica microspheres are easy to prepare (Journal of Chromatography A, 2016, 1471, 138-144 and Journal of Applied Polymer Science, 2015, 132 (20), 113-114)

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