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Method for determining content of nervonic acid in dried blood filter paper by high performance liquid chromatography-tandem mass spectrometry

A technology of high performance liquid chromatography and dried blood filter paper, applied in the field of high performance liquid chromatography tandem mass spectrometry to determine the content of nervonic acid in dried blood filter paper, which can solve the problems of long time consumption, high cost, poor stability, etc.

Pending Publication Date: 2022-08-05
昆明和合医学检验所有限公司
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Problems solved by technology

[0003] At present, the detection of nervonic acid content in the human body is mainly derivatization method, liquid chromatography, gas chromatography, liquid chromatography tandem mass spectrometry (serum), all of which are time-consuming, high cost, low sensitivity, and poor stability. And other problems, resulting in low reliability of test results

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  • Method for determining content of nervonic acid in dried blood filter paper by high performance liquid chromatography-tandem mass spectrometry
  • Method for determining content of nervonic acid in dried blood filter paper by high performance liquid chromatography-tandem mass spectrometry
  • Method for determining content of nervonic acid in dried blood filter paper by high performance liquid chromatography-tandem mass spectrometry

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[0027] The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0028] The present invention provides a detection method for the determination of nervonic acid content in dried blood filter paper by high-performance liquid chromatography tandem mass spectrometry, which is characterized by comprising the following steps:

[0029] 1: Preparation of standard solution: Accurately weigh 5mg of nervonic acid standard in a 10mL volumetric flask, dissolve in pure methanol to obtain standard mother solution A1 (concentration 500ug / mL), and dilute to 0.078125ug / mL, 0.3125ug / mL, 1.25 Nervous a...

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Abstract

The method for detecting the content of nervonic acid in the dried blood filter paper by liquid chromatography tandem mass spectrometry comprises the following steps: carrying out constant-temperature oscillation extraction on an unknown nervonic acid sample through methanol / acetonitrile (with the volume of 1: 1), centrifuging, taking supernatant, carrying out nitrogen blowing redissolution, separating nervonic acid through liquid chromatography, carrying out APCI ionization, and detecting the content of nervonic acid in the dried blood filter paper by using the liquid chromatography tandem mass spectrometry. Performing quantitative analysis on nervonic acid characteristic ion peaks 367.3 / 349.45 through triple quadrupole mass spectrometry, drawing a standard curve by taking the concentration of a standard substance as an abscissa and the peak area of the standard substance as an ordinate, and calculating the content of nervonic acid in the to-be-detected sample. The detection method is small in required sample size, high in sensitivity, strong in specificity and good in reproducibility, the detection time can be remarkably shortened, and a reliable basis can be provided for clinical diagnosis.

Description

technical field [0001] The invention relates to the field of medical testing, in particular to a method for determining the content of nervonic acid in dried blood filter paper by high performance liquid chromatography tandem mass spectrometry. Background technique [0002] Nervous acid (NA) is an ultra-long-chain monounsaturated fatty acid. It is named nervonic acid because it was found in mammalian nerve tissue early. Nervous acid has a high content in the brain and nerve tissue, and is the core natural component of brain nerve fibers and nerve cells. Preliminary studies have shown that it has the function of promoting the development of myelin in the infant brain. The lack of nervonic acid will cause cerebral diseases such as stroke sequelae, senile dementia, cerebral palsy, brain atrophy, memory loss, insomnia and forgetfulness. In addition, according to the latest research, since nervonic acid is the only substance that can repair nerve myelin sheaths, many of the eff...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8634
Inventor 吕小波黄和飞谷叶李佳兴何水兰
Owner 昆明和合医学检验所有限公司
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