Application of carragheen and starch as insect feed coagulant
A technology of artificial feed and Noctuidae, which is applied in the field of coagulant for artificial insect feed, can solve the problems of changing the nutritional ratio of feed, and achieve the effect of reducing costs
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Embodiment 1
[0014] Example 1: Effects of Different Coagulant Components on the Growth and Development of Insects
[0015] In addition to the coagulant, the formula of the feed is as follows:
[0016] Wheat bran 10g, soybean powder 8g, yeast powder 6g, sorbic acid 0.1g, ethyl p-hydroxybenzoate 0.1g, vitamin C 0.2g.
[0017] The ratio of coagulant components and water is shown in Table 1. The starch was potato starch.
[0018] Mix the weighed soybean powder, wheat bran, yeast powder, carrageenan and starch with water. Mix the ingredients evenly, sterilize at a high temperature of 0.15 MPa for 30 minutes, take it out, add sorbic acid, ethyl p-hydroxybenzoate and vitamin C, stir well and let it cool to room temperature.
[0019] Above-mentioned each group feed is cut into thin slice of uniform size respectively (make larva eat up about one day), put into diameter 3cm, in high-temperature sterilized circular plastic film box of 5cm, then film box is placed in a long 30cm , 28cm in width an...
Embodiment 2
[0061] Embodiment two: Carrageenan and starch are used as the influence of coagulant on beet armyworm virus output
[0062] Except for the coagulant, the rest of the ingredients and the preparation method of the feed were the same as in Example 1.
[0063] The ratio of coagulant and water is shown in Table 3.
[0064] Coagulant composition and water consumption in table 3 feed
[0065] Feed number Carrageenan (g) Starch (g) Water (ml) Agar (g)
[0066] 17 1.0 2 90 ——
[0067] 18 1.4 0 80 ——
[0068] 19 1.8 4 100 ——
[0069] 20 1.2 2 100 ——
[0070] Control feed -- -- 100 1.6
[0071] Cut each of the above-mentioned feeds evenly into small slices (the amount of feed should be such that the larvae can finish eating within one day), put them into a high-temperature sterilized plastic film box with a diameter of 3 cm and a height of 5 cm, and use a 100 μl pipette to Draw 10μl2.6×10 6 The beet armyworm nucleopolyhedrosis virus liquid (SeNPV) of PIB / ml is evenly sprayed on t...
Embodiment 3
[0095] Example 3: Comparison of the effects on SeNPV proliferation of carrageenan and starch mixed with optimized ratio and agar as the coagulant. The ratio of the coagulant and water in the feed is shown in Table 5.
[0096] table 5
[0097] Feed number Carrageenan (g) Starch (g) Water (ml) Agar (g)
[0098] 21 1.4 2 110 0
[0099] Control feed -- -- 100 1.6
[0100] All the other ingredients of feed and the preparation of feed are with embodiment one.
[0101] Except that the larvae of beet armyworm used were 4th instar, all the other experimental procedures were the same as in Example 2.
[0102] The experimental results are shown in Table 6.
[0103] The comparison of No. 621 diet and control diet on the proliferation of SeNPV
[0104] Infection Mortality Average SeNPV content per head SeNPV Total SeNPV content per gram of worm carcasses
[0105] feed number
[0106] (%) (×10 8 PIB / head) (×10 8 PIB / g) (×10 8 PIB)
[0107] 21 87.8±6.9aA 20....
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