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Growth hormone liposome for pig and its preparation method

A growth hormone and liposome technology, applied in the field of biomedicine, can solve the problems of difficulty in large-scale promotion, time-consuming and labor-intensive, and difficult to use, and achieve the effects of overcoming instability, increasing daily increment, and facilitating transportation.

Inactive Publication Date: 2004-08-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The above three methods are time-consuming and labor-intensive, and require surgery, which are difficult to use and difficult to promote on a large scale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Take by weighing lecithin 0.7325g, cholesterol 0.1474g, octadecylamine 0.0326g, transfer to the flask at the bottom of the garden;

[0033] (2) Add 30ml ether and wait for it to dissolve completely;

[0034] (3) Add 5mgPST and dissolve it in 25mlPBS, and pour it into a round bottom flask;

[0035] (4) Ultrasound for 5 minutes;

[0036] (5) Evaporate under reduced pressure until gelatinous, and form a film, add 25ml of PBS to wash the film completely;

[0037] (6) Evaporate under reduced pressure to extract ether completely;

[0038] (7) Freeze-dry at -65--70°C for 3-4 days, and then obtain the white powder needle crystal PST liposome product after freeze-drying.

Embodiment 2

[0040] (1) Weigh 2.918g of lecithin, 0.589g of cholesterol, 0.134g of octadecylamine, 5.84g of trehalose, V E 14.59 g, transferred to a round bottom flask;

[0041] (2) Add 80ml of ether and wait for it to dissolve completely;

[0042] (3) Add 0.020g PST and dissolve it in 100ml PBS, pour it into a round bottom flask;

[0043] (4) 40% intensity ultrasound for 5 minutes;

[0044] (5) After decompression treatment at 30°C, add 120ml of PBS to wash the membrane completely, and then decompression treatment to remove the remaining trace solvent;

[0045] (6) After standing for 30 minutes, put it in a -65-70°C refrigerator for 3-4 days. get the product.

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PUM

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Abstract

The present invention relates to a growth hormone liposome for pig and its preparation method. It is a new preparation type of growth hormone slow-release preparation for pig, and its preparation method is characterized by that it uses the bimolecular layer formed from lipoid substance as membrane material to cover growth hormone for pig so as to obtain the invented product. The main components of said membrane material are lecithin, cholesterol and n-octadecylamine. Said invented product is high in covering rate, possesses long-acting slow-releasing action, and the slow release time in pig body is greater or equal to 30 days. Said invention adopts injection method, is simple and easy to implement.

Description

technical field [0001] The invention relates to a porcine growth hormone liposome and a preparation method thereof. The somatotropin liposome for porcine is a new type of porcine somatotropin sustained-release preparation, which belongs to the technical field of biomedicine. Background technique [0002] Growth hormone (Growth Hormone or Somatotropin, ST) is a polypeptide hormone produced by eosinophilic cells in the anterior pituitary gland of animals. It can improve the productivity of livestock, and has a significant and stable effect on increasing production in pigs. In recent years, using biotechnology, porcine somatotropin (PST) has been able to be commercially produced, and its use in pig farming will greatly affect production technology, economic benefits and consumption patterns. [0003] At present, there are three types of growth hormone preparations and application techniques. [0004] 1. Make a buffer and use the injection method. [0005] Store the somatotro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K38/27A61P43/00
Inventor 胥传来乐国伟李相前姚惠源詹斌施用辉朱建平孟旭
Owner JIANGNAN UNIV
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