Growth hormone liposome for pig and its preparation method
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A growth hormone and liposome technology, applied in the field of biomedicine, can solve the problems of difficulty in large-scale promotion, time-consuming and labor-intensive, and difficult to use, and achieve the effects of overcoming instability, increasing daily increment, and facilitating transportation.
Inactive Publication Date: 2004-08-04
JIANGNAN UNIV
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0010] The above three methods are time-consuming and labor-intensive, and require surgery, which are difficult to use and difficult to promote on a large scale
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0032] (1) Take by weighing lecithin 0.7325g, cholesterol 0.1474g, octadecylamine 0.0326g, transfer to the flask at the bottom of the garden;
[0033] (2) Add 30ml ether and wait for it to dissolve completely;
[0034] (3) Add 5mgPST and dissolve it in 25mlPBS, and pour it into a round bottom flask;
[0035] (4) Ultrasound for 5 minutes;
[0036] (5) Evaporate under reduced pressure until gelatinous, and form a film, add 25ml of PBS to wash the film completely;
[0037] (6) Evaporate under reduced pressure to extract ether completely;
[0038] (7) Freeze-dry at -65--70°C for 3-4 days, and then obtain the white powder needle crystal PST liposome product after freeze-drying.
Embodiment 2
[0040] (1) Weigh 2.918g of lecithin, 0.589g of cholesterol, 0.134g of octadecylamine, 5.84g of trehalose, V E 14.59 g, transferred to a round bottom flask;
[0041] (2) Add 80ml of ether and wait for it to dissolve completely;
[0042] (3) Add 0.020g PST and dissolve it in 100ml PBS, pour it into a round bottom flask;
[0043] (4) 40% intensity ultrasound for 5 minutes;
[0044] (5) After decompression treatment at 30°C, add 120ml of PBS to wash the membrane completely, and then decompression treatment to remove the remaining trace solvent;
[0045] (6) After standing for 30 minutes, put it in a -65-70°C refrigerator for 3-4 days. get the product.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The present invention relates to a growth hormone liposome for pig and its preparation method. It is a new preparation type of growth hormone slow-release preparation for pig, and its preparation method is characterized by that it uses the bimolecular layer formed from lipoid substance as membrane material to cover growth hormone for pig so as to obtain the invented product. The main components of said membrane material are lecithin, cholesterol and n-octadecylamine. Said invented product is high in covering rate, possesses long-acting slow-releasing action, and the slow release time in pig body is greater or equal to 30 days. Said invention adopts injection method, is simple and easy to implement.
Description
technical field [0001] The invention relates to a porcine growth hormone liposome and a preparation method thereof. The somatotropin liposome for porcine is a new type of porcine somatotropin sustained-release preparation, which belongs to the technical field of biomedicine. Background technique [0002] Growth hormone (Growth Hormone or Somatotropin, ST) is a polypeptide hormone produced by eosinophilic cells in the anterior pituitary gland of animals. It can improve the productivity of livestock, and has a significant and stable effect on increasing production in pigs. In recent years, using biotechnology, porcine somatotropin (PST) has been able to be commercially produced, and its use in pig farming will greatly affect production technology, economic benefits and consumption patterns. [0003] At present, there are three types of growth hormone preparations and application techniques. [0004] 1. Make a buffer and use the injection method. [0005] Store the somatotro...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more