A kind of antibody fusion protein targeting frizzled-7 and its preparation method and application

A fusion protein and antibody technology, applied in the field of bioengineering, can solve the problems of patient antibody response difference, affinity difference, decline, etc., and achieve the effect of preventing tumor immune escape
CN112159477BActive Publication Date: 2021-09-24SHANGHAI UNIV OF MEDICINE & HEALTH SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANGHAI UNIV OF MEDICINE & HEALTH SCI
Publication Date
2021-09-24

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Abstract

The invention relates to an antibody fusion protein targeting Frizzled-7, a preparation method and application thereof, and belongs to the field of bioengineering. Compared with the prior art, the present invention fuses and expresses the Fzd7-targeting humanized antibody SHH002-hu1 and MICA protein through genetic engineering. This design combines Fzd7-targeting therapy with immune activation for the first time, and utilizes the antibody of the fusion protein In part, it binds to tumor cells positively expressing Fzd7, and anchors MICA molecules on the surface of tumor cells; then uses MICA to specifically bind NKG2D, the surface membrane receptor of NK cells, to gather NK cells to tumor lesions and activate NK cells to play an important role in tumor cells. The killing effect of the antibody can avoid the immune escape of the tumor in conventional antibody therapy, and at the same time enhance the anti-tumor effect of the antibody.
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Description

technical field

[0001] The invention belongs to the field of bioengineering, and in particular relates to an antibody fusion protein targeting Frizzled-7 and its preparation method and application. Background technique

[0002] Natural killer (NK) cells are cells of the innate immune system that exert cytotoxicity, play a key role in tumor immune surveillance, and are an important tool in tumor immunotherapy. The activation of NK cells is regulated by the balance between activating receptors and inhibitory receptors, and the main activating receptors of NK cells include Fc fragment receptor (FcγRIIIa) and NKG2D. A conventional drug for activating and rebuilding the body's tumor immunity is an antibody. Most of the anti-tumor antibodies widely used in clinical practice are IgG1 type, which can activate NK cells through the combination of Fc fragment and FcγRIIIa to exert antibody-dependent cell-mediated cytotoxicity. (antibody-dependent cell-mediated cytotoxicity, ADCC). Ho...

Claims

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