Low-intensity focused ultrasound response type phase-change thrombolysis nano particles as well as application and preparation method thereof

A focused ultrasound and nanoparticle technology, applied in the field of biomedicine, can solve the problems of low strength, limited drug loading, poor thrombolysis effect, etc., achieve good stability, good encapsulation and film formation, and improve biological safety. sexual effect

Active Publication Date: 2019-02-12
THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] One of the purposes of the present invention is to provide a low-intensity focused ultrasound-responsive phase-change thrombolytic nanoparticle to sol

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  • Low-intensity focused ultrasound response type phase-change thrombolysis nano particles as well as application and preparation method thereof
  • Low-intensity focused ultrasound response type phase-change thrombolysis nano particles as well as application and preparation method thereof
  • Low-intensity focused ultrasound response type phase-change thrombolysis nano particles as well as application and preparation method thereof

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Experimental program
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Embodiment Construction

[0056] The following is further described in detail through specific implementation methods:

[0057] One, the preparation method of the low-intensity focused ultrasound response type phase-change thrombolytic nanoparticles of the present invention, the specific steps are as follows:

[0058] (1) Preparation of Fe 3 o 4 -PLGA-PFH nanoparticles

[0059] Weigh 50mg of PLGA and dissolve in 2mL of dichloromethane, and add 0.2mL of PFH and oleic acid-modified Fe 3 o 4 The solution was emulsified for 60 s with an ultrasonic breaker, then 5 mL of 4% PVA solution was added, and the emulsification was continued for 60 s, and 10 mL of 2% isopropanol solution was added and magnetically stirred for 3 h under ice bath conditions. After centrifugation and washing, the Fe 3 o 4 - PLGA-PFH nanoparticles.

[0060] (2) Preparation of Fe 3 o 4 -PLGA-PFH-CREKA nanoparticles

[0061] Fe prepared in step (1) 3 o 4 - PLGA-PFH nanoparticles are dissolved in pH5.2, 0.1M MES buffer, appropr...

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Abstract

The invention relates to the technical field of biomedicine and discloses low-intensity focused ultrasound response type phase-change thrombolysis nano particles which comprise shell polylactic acid-glycolic acid copolymer PLGA, wherein ferrosoferric oxide Fe3O4 is embedded into the shell; perfluorohexane PFH is coated in the shell; the shell is connected with CREKA polypeptide targeting fibrin. The low-intensity focused ultrasound response type phase-change thrombolysis nano particles can solve the problem of an unsatisfactory thrombolysis effect caused by limited drug loading capacity or drug inactivity of drug-loading nano particles in the prior art.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a low-intensity focused ultrasound responsive phase-change thrombolytic nanoparticle, its application and its preparation method. Background technique [0002] A thrombus is a small piece of blood that forms on the surface of a vessel in the cardiovascular system where the lining has been peeled off or repaired. In variable flow dependent patterns, thrombi consist of insoluble fibrin, deposited platelets, accumulated leukocytes, and trapped erythrocytes. [0003] Thrombosis is the main pathogenesis of acute ischemic cardiovascular disease and is the main cause of unstable angina, myocardial infarction and stroke. For a long time, because recombinant tissue plasminogen activator (rtPA) can activate plasminogen, and then dissolve thrombus, it is a commonly used drug in the field of thrombolysis, so patients with thrombosis mainly use recombinant tissue plasminogen activator (r...

Claims

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Application Information

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IPC IPC(8): A61K49/22A61K49/18A61K49/12A61K41/00A61K9/51A61K47/34A61K47/18A61P7/02
CPCA61K9/5123A61K9/5153A61K41/0028A61K49/12A61K49/1878A61K49/225A61P7/02
Inventor 钟毅欣郭大静周君徐杰叶曼何晓静宋卫香候静馨刘佳
Owner THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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