L-arginine producing strain and its mutation method and usage in producing L-arginine

Abstract

The present invention relates to the fermentation process of producing L-arginine. By using Corynebacterium crenatum SYA5 screened and preserved by the present lab as parent and through conventional physical and chemical mutation process and multiple structural analog resistance screening, mutant strain SDNN403 is obtained. Through culturing of the mutant strain under optimized condition, L-arginine is produced in the yield level of 30-35 g / L. The strain has high genetic stability, stable yield characteristic, high L-arginine yield level, less produced hetero acids and easy technological amplification, and is suitable four industrial production.

Description

technical field

[0001] A bacterial strain producing L-arginine, its mutagenesis method and a method for producing L-arginine using the bacterial strain. The present invention relates to using glucose and ammonium sulfate as main raw materials and utilizing Corynebacterium crenatum to mutate A method for producing L-arginine by strain fermentation. Background technique

[0002] L-arginine is a semi-essential amino acid for human body and animals. It has important biochemical and physiological significance, so it is widely used in food and pharmaceutical industries, especially in the field of pharmaceutical industry. Medicines for the treatment of vascular diseases and enhancing sperm motility in men have received attention.

[0003] The preparation methods of L-arginine include extraction method (Chinese patent CN1161959) and fermentation method. The extraction method is time-consuming, has low yield, serious environmental pollution, and is not suitable for large-scale produ...

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