Method for identifying i(mycobacterium) species and diagnosing reagent kit therefor

A technology for mycobacteria and mycobacterial antigens, which can be used in biochemical equipment and methods, analytical materials, and microbial determination/inspection, etc., and can solve problems such as the specificity of protein antigens.

Inactive Publication Date: 2004-12-22
RAPID MEDICAL DIAGNOSTICS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The focus of these attempts has been mainly on the discovery of species-specific glycolipid antibodies for the detection of specific humoral immune responses because of the problematic specificity of protein antigens

Method used

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  • Method for identifying i(mycobacterium) species and diagnosing reagent kit therefor

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Experimental program
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Embodiment

[0034] Preparation of KP90 / KS90

[0035] KP90 is prepared with starting materials derived from crude mycobacterial substance, as described in WO-A-94 / 14069 (the content of this invention is incorporated herein by reference). The starting material prepared with 1 gram of freeze-dried bacteria was centrifuged at 90,000×g at 4°C for 2 hours. The pellet was washed twice with PBS. Between washing steps, the samples were sonicated on ice for 6×30 seconds with 10 seconds interruption each time, and then centrifuged at 90,000×g, 4°C for 2 hours. Collect the pellet and resuspend it in 10 ml of 0.05M Tris buffer (TBS) pH 7.4. The supernatant designated as KS90 can be used as an antigen. The precipitate was sonicated on ice for 6×15 seconds, with 10 seconds interruption each time. This formulation is designated the term KP90. After determining the protein concentration, 1.7 ml was lyophilized at a concentration of 1 mg / ml (dissolved in 25mMTBS / 50% glycerol) and stored at -20°C. The following...

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Abstract

The invention relates to a method for identifying a Mycobacterium species, comprising the steps of: a) contacting at least one immuno-cross reactive antigen component of a mycobacterial species with a sample of a body fluid of a human or animal individual; b) contacting at least one antibody, which is capable of reacting with a mycobacterial antigen, with said body fluid sample; c) detecting the presence of antigen-antibody complexes, and identifying the Mycobacterium species present in said body fluid sample.

Description

Technical field [0001] The present invention relates to a method for identifying mycobacteria causing human or animal mycobacterium infection, and a diagnostic kit for the above method. Background technique [0002] Mycobacterium (Mycobacterium) contains 50 species. It is the cause of many diseases collectively referred to as mycobacterial disease. The most famous and most widespread of these diseases are leprosy caused by Mycobacterium leprae and tuberculosis caused by Mycobacterium tuberculosis. These two diseases have infected more than 10 million people worldwide. Most other mycobacteria normally exist only in the form of environmental saprophytes. However, they may also cause opportunistic diseases, which often (but not alone) occur in organisms with a problematic immune system, such as AIDS patients or immunosuppressed patients. Opportunistic types include the slow-growing species Mycobacterium avium (M.Avium), and the closely related Mycobacterium intracellulare (M.Intrace...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N33/569
CPCG01N33/5695
Inventor H·-J·豪图夫S·克龙斯沃特R·范德尔默伦S·格达雅尔A·科尔克L·佩雷拉阿里亚斯布达S·克伊珀
Owner RAPID MEDICAL DIAGNOSTICS CORP
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