Compound of phycocyania of blue-green algae, its preparing method and usage
A compound and material technology, applied in the field of violacyanin compound, its preparation and application, can solve problems such as unsatisfactory effects, and achieve the effects of increasing the content of violacyanin active ingredients, improving physical properties, and stabilizing the process
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Embodiment 1
[0059] Embodiment 1: the preparation of violacein
[0060] 1. Plant material handling
[0061] Take 100 g of fresh leaves of Peristrophe baphia (Spreng.) Bremek. [Peristropheroxbueghiana (schult) Bremek]), add 400 ml of 1% formic acid, mash them, add silica bath soil filter aid, and obtain the extract. XAD-16 resin column, eluted with 0.1% formic acid to obtain eluent F001. F001 Contains Zilanaminol
[0062] 2. Alkali treatment
[0063] The eluent F001 was basified with dilute ammonia water (0.1%) until blue and purple appeared, and the basified solution F002 was obtained, and violamin was transformed into violacyanin.
[0064] 3. Concentrate
[0065] Concentrate the alkalized solution F002 under vacuum below 60°C to obtain the concentrated solution F003.
[0066] 4. Column chromatography
[0067] Put the concentrated solution on a C18 bonded phase silica gel column (40-60 μ), elute with water and 30% methanol gradient, collect the eluent F004 of the color band, concentr...
Embodiment 2
[0076] Embodiment 2: the preparation of violacein
[0077] 1. Plant material handling
[0078] Take 100 g of fresh branches and leaves of Peristrophe baphia (Spreng.) Bremek. [Peristropheroxbueghiana (schult) Bremek]), put them in a 500ml round bottom flask, and blow off the air with nitrogen gas. Quickly pour 400ml of boiling water that has boiled for 3 minutes (to remove dissolved oxygen), place it in a water bath of 86°C, turn the flask to make the temperature uniform, and directly contact the boiling water with the indigo material at room temperature (22°C) to make the material in 2 Within minutes, the temperature is raised to 80°C, and the mixed material is kept at about 86°C for 90 minutes. Pour out the extract and take out the slag. Add 400ml of water to the slag, boil and extract for 10 minutes, filter, and repeat the extraction 3 times. Combine above-mentioned 4 parts extracts, obtain 1758ml feed liquid (T001).
[0079] 2. Separation and purification of violacein ...
Embodiment 3
[0089] Embodiment 3: the influence of heating rate
[0090] Take 50g of fresh mountain indigo, put it in a 500mL conical flask, and inject N 2 , Add 400 mL of boiling deoxygenated distilled water. Divided into rapid heating group and controlled heating group. The rapid temperature rise test uses 89°C hot water to quickly mix with materials (room temperature 22°C) (the sample bottle is preheated in an 82°C water bath about 0.7 minutes before mixing), and the materials can reach an equilibrium temperature of about 82°C once they are mixed. In this way, the time for the material to go from 50°C to 70°C is almost 0, and it is kept in an 82°C water bath for 110 minutes. In the controlled temperature rise test, cold water is mixed with the material, the heating rate is controlled from 50°C to 70°C under stirring, the material mixture is heated to 82°C, and kept in an 82°C water bath for 110 minutes. Pour out the feed liquid, extract the residue 3 times again, and set the volume t...
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