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Compound of phycocyania of blue-green algae, its preparing method and usage

A compound, the technology of Violet Blue, which is applied in the direction of active ingredients of heterocyclic compounds, food preparation, application, etc., can solve the problems of unsatisfactory effects, etc., and achieve the improvement of content of active ingredients of Violet Blue, product quality, and good quality Effect

Inactive Publication Date: 2003-07-23
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Natural medicines include glycyrrhizin and silymarin, and traditional Chinese medicines include phylloxera, cress, myrobalan and other single medicines and various compound Chinese medicines, but the effect is not satisfactory, and there is an urgent need to provide new effective medicines for treating hepatitis B

Method used

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  • Compound of phycocyania of blue-green algae, its preparing method and usage
  • Compound of phycocyania of blue-green algae, its preparing method and usage
  • Compound of phycocyania of blue-green algae, its preparing method and usage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Embodiment 1

[0054] The following are non-limiting examples of the present invention Example 1: Preparation of Violet

[0055] 1. Plant material handling

[0056] Take 100 g of fresh leaves of Peristrophe baphia (Spreng.) Bremek. [Peristropheroxbueghiana (schult) Bremek]), add 400 ml of 1% formic acid, mash them, add silicon bath soil filter aid, and obtain the extract. XAD-16 resin column, eluted with 0.1% formic acid to obtain eluent F001. F001 Contains Zilanaminol

[0057] 2. Alkali treatment

[0058] The eluent F001 was basified with dilute ammonia water (0.1%) until blue and purple appeared, and the basified solution F002 was obtained, and violamin was transformed into violacyanin.

[0059] 3. Concentrate

[0060] Concentrate the basified solution F002 under vacuum below 60°C to obtain the concentrated solution F003.

[0061] 4. Column chromatography

[0062] Put the concentrated solution on a C18 bonded phase silica gel column (40-60 μ), elute with water and 30% methanol gradie...

Embodiment 2

[0066] Violet blue pigment: black amorphous solid. C 18 h 17 N 3 o 8 (Molecular weight M=403), ultraviolet-visible spectrum (UV-Vis) figure sees figure 1 , the absorption peak λ max (ε): 239 (24925), 477 (17271) nm in pH3.0 0.05mol / glycine-HCl buffer; at pH7.0 0.1mol / L Na 2 HPO 4 235 (shoulder, 23944), 248 (62150), 279 (11580), 319 (8505), 550 (shoulder, 34347), 382 (38926) nm in the buffer; at pH8.0 0.05mol / L trihydroxy In methylaminomethane buffer, 235 (shoulder peak, 23944), 248 (62150), 279 (11580), 319 (7916), 550 (shoulder peak, 35066), 582 (41347) nm. Infrared Spectrum IR(KBr)ν max 806, 844, 876, 938, 993, 1013, 1090, 1131, 1158, 1177, 1204, 1223, 1259, 1284, 1308, 1335, 1370, 1402, 1458, 1477, 1508, 1521, 1572, 1603, 1641, 1680 (shoulder peak), 2500-3600 (broad peak), the spectrum is shown in figure 2 . The NMR spectra of Violet Blue are shown in Table 2. Pseudomolecular ions were measured by matrix-assisted laser desorption ionization time-of-flight mass...

Embodiment 3

[0078] The fine jelly (T006) was dispersed with 50 ml of pyridine-isopropanol (1:9), filtered, and the filter cake was washed with 50 ml of pyridine-isopropanol (1:9), and then washed with 30 ml of isopropanol, and then Washed with 30ml of acetone and dried to obtain 191mg of pure violacein as a black amorphous solid. Embodiment 3: the influence of heating rate

[0079] Take 50g of fresh mountain indigo, put it in a 500mL conical flask, and inject N 2 , Add 400 mL of boiling deoxygenated distilled water. Divided into rapid heating group and controlled heating group. The rapid temperature rise test uses 89°C hot water to quickly mix with materials (room temperature 22°C) (the sample bottle is preheated in an 82°C water bath about 0.7 minutes before mixing), and the materials can reach an equilibrium temperature of about 82°C once they are mixed. In this way, the time for the material to go from 50°C to 70°C is almost 0, and it is kept in an 82°C water bath for 110 minutes. ...

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PUM

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Abstract

A zilanine compound, its preparing process, the medicine composition containing its as active component, and its application in preparing the medicines for treating diabetes and hepatitis B, antioxidizing medicine, immune improver, the health-care product for beautifying face and delaying sanility, and the colouring agent are disclosed.

Description

(1) Technical field: [0001] The present invention relates to a heterocyclic compound, in particular a ring fused to a carbocyclic ring having three nitrogen atoms as the only ring heteroatoms or having two nitrogen atoms and an oxygen atom as the only ring heteroatoms Atom ring heterocyclic compound, the present invention also relates to the preparation method of the compound, the pharmaceutical composition with the compound as an active ingredient, and its use in the prevention and treatment of diabetes, hepatitis B, and as an antioxidant drug and health care product, and use as a coloring agent. (two) background technology: [0002] Diabetes is currently a high-incidence species at home and abroad. The incidence rate in developed countries such as Europe and the United States is as high as 8%, and the incidence rate in my country has reached 3%. There are more than 100 million patients in the world, and more than 40 million patients in my country. In Guangdong alone, there...

Claims

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Application Information

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IPC IPC(8): A23L33/105A61K31/395A61K31/55A61P1/16A61P3/10A61P31/12A61P39/06C07D255/00C07D273/02C07D273/06
Inventor 谢运昌蒋小华文永新程菊英方宏
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI