Urushi polysaccharide extracting method

A technique for raw lacquer and polysaccharide, which is applied in the field of extracting natural lacquer polysaccharide with tumor-inhibiting properties, can solve the problems of being unsuitable for mass production, poor separation and purification efficiency, troublesome grading operation, etc., and achieves low cost, biological activity inhibition, and good purity. Effect

Inactive Publication Date: 2005-09-14
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the disadvantages of low polysaccharide yield (about 1%), poor separation and purification efficiency, and is not suitable for mass production
Using ethanol, acetone system precipitation fractionation or column chromatography fractionation operation is troublesome and the cycle is long

Method used

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  • Urushi polysaccharide extracting method
  • Urushi polysaccharide extracting method
  • Urushi polysaccharide extracting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Add 5 times the amount of acetone to 1kg sumac sap to soak and fully stir. After standing still, polysaccharides and glycoproteins, etc. will precipitate out with gray powder, pour off the black acetone solution, and repeatedly wash the precipitate with acetone several times until the acetone washes out Nearly colorless. The acetone solution was removed by suction filtration, and the precipitate was quickly dried at room temperature to obtain 102 g of gray acetone powder. Add appropriate amount of distilled water to acetone powder, stir overnight, centrifuge and filter to remove insoluble matter. Filtrate plus (NH 4 ) 2 SO 4 To saturation, after standing still for a period of time, centrifuge and suction filter to remove the precipitate, the filtrate is desalted by ultrafiltration with 3kDa membrane, and then concentrated by rotary evaporation at 50°C under reduced pressure, then add absolute ethanol to precipitate the polysaccharide, P 2 o 5 Vacuum-drie...

Embodiment 2

[0017] Example 2: Add appropriate amount of distilled water to the above acetone powder, add ammonium sulfate to 30% (w / w), stir overnight, then centrifuge and suction filter to remove insoluble matter. The filtrate was desalinized with a 3kDa membrane ultrafiltration, and passed through 732 (H + Type) cation exchange resin column, flow rate 10-15ml / h, phenol-sulfuric acid method to detect and collect the polysaccharide solution, neutralize it with dilute NaOH solution to pH 6-7, use 3kDa membrane ultrafiltration to concentrate the solution, and then concentrate under reduced pressure below 50°C , ethanol precipitation, P 2 o 5 The pure lacquer polysaccharide was obtained by vacuum drying with a yield of 5.44%.

Embodiment 3

[0018] Example 3: Add an appropriate amount of distilled water to the above acetone powder, add ammonium sulfate to saturation, stir overnight, centrifuge and filter to remove insoluble matter. The filtrate was desalinized with a 3kDa membrane ultrafiltration, and passed through 732 (H + Type) cation exchange resin column, flow rate 30-45ml / h, phenol-sulfuric acid method to detect and collect polysaccharide solution, neutralize it with dilute NaOH solution to pH 6-7, use 3kDa membrane ultrafiltration to concentrate the solution, and then concentrate under reduced pressure below 50°C , ethanol precipitation, P 2 o 5 The pure lacquer polysaccharide A was obtained by vacuum drying with a yield of 6.56%. The water-soluble glycoprotein absorption peak at 230-270nm in the pure polysaccharide UV spectrum disappears (attached figure 1 ), indicating a high purity polysaccharide.

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Abstract

The invention discloses a process for extracting natural CPS-M having tumor inhibiting function comprising, preparing powder from natural lacquer liquid by propanone or xylene, lixiviating the powder with leach, removing glucoprotein and enzyme through salting out, centrifugal filtration, desalinizing the filter liquor through ultrafiltration or dialysis, column chromatography, neutralizing the chromatography liquid, concentrating, alcohol deposition, vacuum drying. The polysaccharide obtained has evident biological activity for inhibiting S-180 tumor.

Description

technical field [0001] The invention relates to a method for extracting natural lacquer polysaccharides capable of inhibiting tumors. Background technique [0002] Polysaccharides widely exist in living organisms, and their structural diversity enables them to carry a large amount of information and participate in many processes of life. Studies in recent years have shown that polysaccharides have a variety of biological activities, such as anti-tumor, anti-virus, anti-coagulation, anti-aging, anti-angiogenesis, activation of complement or anti-complement and lipid-lowering functions. Polysaccharides with different structures have their special biological activities. However, polysaccharides extracted from plants, animals and fungi often limit their research, development and application due to their low yield. [0003] Raw lacquer polysaccharide is the main component in lacquer liquid, it has the effect of promoting white blood cell growth and other immune aspects, and it ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 杜予民杨建红
Owner WUHAN UNIV
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