Method for soaking oil from cotton seed embryo piece and producing separation protein
A technology for separating protein and cottonseed germ flakes, which is applied in the direction of fat oil/fat production, protein composition of vegetable seeds, protein food ingredients, etc. It can solve the problems of complex filtering equipment, large filtering equipment, high desolvation temperature, etc., and achieve equipment layout Reasonable, simple and mature process, high protein quality effect
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Embodiment 1
[0015] Example 1: Cottonseed germ slices were immersed in No. 4 solvent to obtain mixed oil and wet meal. The temperature during oil immersion was 20°C. The mixed oil was evaporated to obtain cottonseed crude oil, and the solvent was recovered, and the wet meal was desolvated to obtain dry meal. The desolvation temperature is 40°C, the pressure is -0.085MPa, and the solvent is recovered. The dry meal is subjected to alkali dissolution and a centrifugation to obtain a high solid phase and supernatant (I). The temperature during alkali dissolution is 50°C, and the pH value is 9. The solid phase is dried to obtain feed protein, and the supernatant (I) is subjected to acid precipitation, secondary separation, neutralization, homogeneous sterilization, and spray drying to obtain isolated protein. The pH is 4.5 during acid precipitation, and the pH is 4.5 during neutralization. 7. Homogenization sterilization is instant sterilization, and the temperature is 125°C.
Embodiment 2
[0016] Example 2, the difference between Example 2 and Example 1 is that the temperature during oil immersion is 60°C, the desolvation temperature is -5°C, the pressure is -0.095MPa, the temperature during alkali dissolution is 35°C, and the pH value is 12. The pH value is 3.5 during acid precipitation and 6.5 during neutralization, and the homogenization sterilization is instant sterilization at a temperature of 120°C.
Embodiment 3
[0017] Example 3, the difference between Example 3 and Example 1 is that the temperature during oil immersion is 0°C, the desolvation temperature is 60°C, the pressure is -0.01MPa, the temperature during alkali dissolution is 65°C, the pH value is 8, and the acid The PH value is 5.5 when sinking, and 7.5 when neutralizing. The homogenization sterilization is instantaneous sterilization, and the temperature is 140°C.
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