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Amnia preservation and handling method

A processing method and technology for amniotic membranes, which are applied in the preservation of human or animal bodies, medical raw materials from mammals, animal husbandry, etc., can solve the problems of inconvenient transportation, short storage time of amniotic membranes, and cannot guarantee urgent clinical needs, etc., and achieve alleviation. Clinical stress, convenient transportation effect

Inactive Publication Date: 2006-02-15
JIANGXI RUIJI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fresh amniotic membranes are often not guaranteed for urgent clinical needs, and the storage time of amniotic membranes stored at low temperature is short, with a maximum storage time of 1 to 2 months, and transportation is inconvenient

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] The placental tissue of healthy cesarean section mothers was selected, and prenatal serological examination was carried out in accordance with relevant national standards to exclude hepatitis B, hepatitis C, syphilis and acquired immunodeficiency syndrome. The amniotic membrane and chorion of the placenta were bluntly peeled off, washed, and the obtained amniotic epidermal cell layer was placed on the top, and the bottom was held by nitrocellulose filter paper, immersed in 8% glycerin, and the material was placed in the working room through a freeze dryer. Pre-freeze at 0°C to -30°C for 1 to 2 hours, turn on the condensing chamber for refrigeration, and turn on the vacuum pump when the temperature of the cold trap reaches -35°C to -45°C, and vacuum freeze-dry for 3 to 8 hours. The dried amnion is packaged and extinguished. Bacteria can be stored at room temperature.

Embodiment 2

[0010] The placental tissue of healthy cesarean section mothers was selected, and prenatal serological examination was carried out in accordance with relevant national standards to exclude hepatitis B, hepatitis C, syphilis and acquired immunodeficiency syndrome. The amnion and chorion of the placenta were bluntly peeled off, washed, and the obtained amnion epidermal cell layer was on the top, and the bottom was held by nitrocellulose filter paper, immersed in 10% glycerin, and the material was placed in the working room by a freeze dryer. Pre-freeze at 0°C to -30°C for 1 to 2 hours, turn on the condensing chamber for refrigeration, and turn on the vacuum pump when the temperature of the cold trap reaches -35°C to -45°C, and vacuum freeze-dry for 8 hours. The dried amniotic membrane is packaged and sterilized. Can be stored at room temperature.

Embodiment 3

[0012] The placental tissue of healthy cesarean section mothers was selected, and prenatal serological examination was carried out in accordance with relevant national standards to exclude hepatitis B, hepatitis C, syphilis and acquired immunodeficiency syndrome. The amniotic membrane and chorion of the placenta were bluntly peeled off, washed, and the obtained amniotic epidermal cell layer was placed on the top, and the bottom was held by nitrocellulose filter paper, immersed in 0.1% glycerin, and the material was placed in the working room through a freeze dryer. Pre-freeze at 0°C to -30°C for 2 hours, turn on the condensation chamber for refrigeration, and turn on the vacuum pump when the temperature of the cold trap reaches -35°C to -45°C, and vacuum freeze-dry for 5 hours. The dried amniotic membrane can be packaged and sterilized. Store at room temperature.

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PUM

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Abstract

The present invention discloses a biological amniotic membrane storage method. Said method includes the following steps: inactively stripping amniotic membrane and chorionic membrane of placenta, cleaning, using nitrocellulose filtering paper to support amniotic epidermal cell layer, using 0.1-10% glycerin to protect, adopting freeze-drying process, prefreezing the material for 1-2 hr. at zero deg.C- -30 deg.C in working room, starting condensing chamber to make refrigeration, when the temp. of cold trap is reached to -35 deg.C- -45 deg.C, starting vacuum pump, vacuum freeze-drying for 3-8 hr., sealing, sterilizing, drying and storing in a cool and dark place so as to implement said invention.

Description

technical field [0001] The invention relates to a method for preserving and processing amniotic membrane raw materials of medical device dressings. Background technique [0002] Due to its unique characteristics, amniotic membrane has attracted the attention of scholars at home and abroad. Amniotic membrane refers to the basement membrane layer of human placenta, which is a collagen tissue layer without blood vessels and cell structure. It is rich in type IV and V collagen and basic fibroblast growth factor. (BFGF), stem cell growth factor (HGF), easy to make epithelial cells migrate into, promote epithelial differentiation and proliferation, and play an important role in epithelial wound healing. Amniotic membrane contains various protein inhibitors, which play an anti-inflammatory effect by inhibiting the corresponding proteases. At the same time, it also contains TGF-β, which inhibits the expression of cytokines and regulates apoptosis 1 , can avoid excessive proliferat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/00A61K35/50
Inventor 苗九昌付筱冲张莉莉饶国厚
Owner JIANGXI RUIJI BIOTECH CO LTD