Human papilloma virus and heat shock protein recombinant protein vaccine and use thereof
A human papilloma virus and recombinant protein technology, applied in the field of human papilloma virus and heat shock protein recombinant protein vaccines and their uses, can solve the problems of no effect, less understanding of the mechanism of action, formation of nodules, etc., and achieve high-efficiency expression , strong immunity, strong anti-tumor function
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[0036] 1. The acquisition and modification of mycobacterial heat shock protein 70 gene, human papillomavirus E6 and E7 genes, and the construction of mE6Δ / mE7 / hsp70Δ fusion gene and recombinant prokaryotic expression plasmid vector
[0037] 1. Acquisition and modification of mycobacterial heat shock protein 70 gene
[0038] Cultivate Mycobacterium tuberculosis, extract genomic DNA, and use PCR to amplify the nucleotide sequence (named TBhsp70Δ) of a part (161-370 amino acids) of hsp70 gene encoding the ATP binding site, and introduce BamH I and Xho at both ends of the sequence. The I restriction enzyme cut site, after sequence analysis, is consistent with the GeneBank comparison sequence.
[0039] 2. Acquisition of human papillomavirus E6 and E7 genes
[0040] Genomic DNA was extracted from the tumor tissues of HPV16-positive cervical cancer patients, and the E6 and E7 genes were amplified by PCR. After sequence analysis, the E7 gene DNA sequence was consistent with foreign report...
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