Artificial T helper cell epitopes as immune stimulators for synthetic peptide immunogens including immunogenic LHRH peptides

A technology of peptide immunogen and immunostimulatory sequence, which is applied in the field of conventional immunostimulatory sequence, and can solve the problems of uneven size and composition

Inactive Publication Date: 2001-10-03
UNITED BIOMEDICAL INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 1. Chemical conjugation is a random reaction leading to inhomogeneity in size and composition, for example, conjugation with glutaraldehyde (Borras-Cuesta et al. Eur. J. Immunol. 1987:17:1213-1215);

Method used

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  • Artificial T helper cell epitopes as immune stimulators for synthetic peptide immunogens including immunogenic LHRH peptides
  • Artificial T helper cell epitopes as immune stimulators for synthetic peptide immunogens including immunogenic LHRH peptides
  • Artificial T helper cell epitopes as immune stimulators for synthetic peptide immunogens including immunogenic LHRH peptides

Examples

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example

[0082] The peptide immunogen of the present invention can be prepared by chemical methods known to those skilled in the art. See, for example, Fields et al., "Synthetic Peptides: User's Manual" Chapter 3, Grant, W.H. Ed., Freeman & Co., New York, NY, 1992, p.77. The peptide can be automatically matched Merrifield solid-phase peptide synthesis method, using t-Boc or Fmoc to protect α-NH 2 Or side chain amino acids, to synthesize. The equipment for peptide synthesis is provided commercially. An example is the 430A or 431 applied biological system peptide synthesizer.

[0083] After completing the assembly of the required peptide immunogen, the resin is processed according to standard procedures to cleave the peptide from the resin and deblock the functional groups on the amino acid side chains. The free peptide is purified by HPLC, and biochemical characteristics are identified by, for example, amino acid analysis, by sequencing, or by mass spectrometry. Methods of purifying and ide...

Embodiment 1

[0103] Immunize rats with peptide immunogen containing LHRH

[0104] The peptides listed in Tables 2a and 2b were synthesized and tested as described below.

[0105] A. Synthesis of peptides. Using Merrifield solid-phase synthesis technology, the peptides listed in Table 2a and 2b were synthesized using Fmoc chemicals on Applied Biosystems' automatic matching peptide synthesizer (Models 430, 431 and 433A). A peptide construct containing the constructed synthetic antigen library (SSAL), for example, the artificial Th site designated as SEQ ID NO: 3, is prepared by providing a mixture of desired amino acids selected for a specific position. After completing the assembly of the required peptide or combination of peptides, the resin is treated with trifluoroacetic acid according to standard procedures to remove the peptide from the resin and deblock the protective groups on the amino acid side chains.

[0106] The excised, extracted and cleaned peptides were purified by HP...

Embodiment 2

[0127] A mixture of LHRH peptides that induces more extensive immune castration in rats

[0128] It was confirmed that the relative effects of the various artificial TH epitopes / LHRH constructs shown in Example 1 can select a more effective peptide mixture that is combined into an enhanced immunogenicity. The Th / LHRH peptide immunogen mixture is more effective than any individual peptide in the mixture of US 5795551. Moreover, a mixture of individual constructs that carry promiscuous Th epitopes derived from MVF Th (SEQ ID NO: 2) and HbsAg Th (SEQ ID NOS: 18-20) provides a greater number of genetically diverse populations than those derived from only one The peptide composition of Th epitope mixed with Th epitope responds more widely. Therefore, a peptide composition containing the peptide mixture of the present invention derived from MVF Th and HbsAg Th was assembled, and the effect of the mixture was tested and compared with the composition containing the individual peptide...

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Abstract

The present invention is directed to novel peptide immunogens for eliciting antibodies to LHRH comprising artificial T helper cell epitopes (Th epitopes) designed to provide optimum immunogenicity. The artificial Th epitopes are covalently linked to LHRH and optionally an immunostimulatory sequence.

Description

Invention field [0001] The present invention relates to a peptide immunogen containing a new artificial T helper cell epitope (Th), which is covalently linked to a desired target antigen site containing a B cell epitope, and optionally A conventional immunostimulatory sequence. This artificial Th epitope confers the peptide immunogen the ability to induce a strong T helper cell-mediated immune response and makes it produce antibodies against the "target antigen site". The present invention also provides the replacement of carrier proteins in established peptide immunosources and T helper cell sites derived from pathogens with this new artificial T helper cell epitope. [0002] Some rules have been formed in predicting the amino acid sequence of T cell epitopes. However, because there is no core unified theory on how or how to prepare a specific amino acid sequence that can be used as a T cell epitope, the existing rules are empirical and do not have universal applicability. On the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/00A61K39/00A61P15/16A61P15/18A61P31/00A61P35/00A61P37/02A61P37/04A61P43/00C07K7/23C07K14/02C07K14/12C07K14/47C07K14/705C07K19/00
CPCC12N2760/18422C12N2760/18722C12N2730/10122C07K7/23A61K39/00C07K14/005A61P15/16A61P15/18A61P31/00A61P35/00A61P37/02A61P37/04A61P43/00
Inventor 王长怡
Owner UNITED BIOMEDICAL INC
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