Recombinant adenovirus for preventing baby's diarrhea

A technology for recombinant adenovirus and infant diarrhea, applied in the field of recombinant adenovirus, can solve problems such as economic loss, and achieve the effects of ensuring safety in use, solving the problem of virus mutation, and being easy to prepare

Inactive Publication Date: 2002-03-06
王健伟 +2
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Rotavirus is the most important cause of diarrhea in infants and young children, and it is widely prevalent in the world. According to the report of the World Health Organization, there were 3 to 5 billion cases of diarrhea in Asian, African, and Latin American countries in 1977-1978. , resulting in 5-10 million child deaths, ranking first in both morbidity and mortality. In another report, it is estimated that in Asian, African, and Latin countries, there are 700-100 million diarrhea cases and 4.6 million deaths each year. Diarrhea, even in developed countries such as the United States, is very harmful. The total expenditure for the treatment of rotavirus infection in the United States exceeds 1 billion U.S. dollars every year. Diarrhea ranks second in the cause of death of infants and young children in my country. From this point of view, rotavirus disease Infected diarrhea not only causes a large number of infant deaths, but also causes economic losses. At present, there is no specific drug for treatment. Therefore, preventing rotavirus infection is an important task to reduce the occurrence of the disease. Therefore, research and development of rotavirus vaccines , can reduce morbidity and mortality, so that infants and young children can reduce the suffering of diarrheal diseases and the threat of death

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  • Recombinant adenovirus for preventing baby's diarrhea
  • Recombinant adenovirus for preventing baby's diarrhea
  • Recombinant adenovirus for preventing baby's diarrhea

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Embodiment Construction

[0025] Cloning of Rotavirus VP6 Gene

[0026] 1. Diarrhea and stool samples were collected from the pediatric outpatient clinic of a domestic hospital, which was confirmed to be rotavirus infection by electron microscopy. Rotavirus genomic RNA was extracted from this sample, and purified as a template. According to the known VP6 gene nucleotide sequence, the upstream and downstream primers were designed, with EcoRI and BamHI restriction sites, respectively, and reverse transcription PCR was used. (RT-PCR) method to amplify the VP6 full-length gene to obtain a fragment with a length of about 1.4Kb. The PCR amplified product was recovered by the low-melting point agarose method, digested by EcoR1 and BamHI, and inserted into the corresponding restriction site of pGEM-7zf vector, and the positive clone pGEM-VP6 inserted into the rotavirus gene was screened out. The sequence analysis results of the positive clones proved that the cloned VP6 gene was completely correct.

[0027] ...

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Abstract

A recombinant adenovirus for preventing baby's diarrhea by preparing its nosal drops, spray, injection and orally applied preparation. It features that its genoma has the deficiency of E1 region (delta E1 ) and E3 region (delta E3) and the gene expression box VP6 of rotavirus is inserted at said deficient positions. Its advantages include high immune effect and safety, low cost and easy storage and preparing.

Description

(1) Technical field [0001] The invention relates to a recombinant adenovirus for preventing diarrhea in infants and belongs to the field of biological genetic engineering. (2) Background technology [0002] Rotavirus is the most important cause of diarrhea in infants and young children, and it is widely prevalent in the world. According to the report of the World Health Organization, there were 3 to 5 billion cases of diarrhea in Asia, Africa, and Latin America in 1977-1978. , resulting in the death of 5-10 million children, ranking first in both morbidity and mortality. In another report, it is estimated that in Asian, African, and Latin American countries, there are 700-100 million diarrhea cases and 4.6 million deaths each year. Diarrhea, even in developed countries such as the United States, is very harmful. The total expenditure for the treatment of rotavirus infection in the United States exceeds 1 billion U.S. dollars every year. Diarrhea ranks second in the cause of de...

Claims

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Application Information

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IPC IPC(8): A61K39/235A61P1/12C12N7/01C12N15/09
Inventor 王健伟何金生洪涛
Owner 王健伟
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