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Method for refrigerating presevation of zhonghua villiform chela crab

A technology of Chinese mitten crab and cryopreservation, which is applied in the direction of animal husbandry and the like

Inactive Publication Date: 2003-08-27
上海金蟹水产科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is that artificial seedlings can be propagated and raised at any time without being restricted by seasons, and a method for cryopreservation of isolated embryos of Chinese mitten crabs is disclosed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0005] Example 1: Peel off the embryo from the abdomen of the ovum-bearing mitten crab, place it in a low-temperature resistant container, and store the container in a freezer at -2°C. After three months, take out the container in the freezer and put it in Thaw in a liquid at 20°C, take the thawed embryos out of the thawed liquid, and put them into an incubator to cultivate larvae. The survival rate of daphnia larvae is over 60%.

Embodiment 2

[0006] Example 2: Peel off the embryos from the abdomen of the ovum-bearing mitten crab, place them in a low-temperature resistant container, and store the container centrally in a freezer at -92°C. After six months, take out the container in the freezer and put it in Thaw in a liquid at 20°C, take the thawed embryos out of the thawed liquid, and put them into an incubator to cultivate larvae. The survival rate of daphnia larvae is over 60%.

Embodiment 3

[0007] Embodiment 3: Peel off the embryo from the abdomen of the ovum-bearing crab, place it in a low-temperature resistant container, and store this container in a cooler at -192°C. After nine months, take out the container in the cooler. Thaw in a liquid at 20°C, take the thawed embryos out of the thawed liquid, and put them in an incubator to cultivate larvae. The survival rate of daphnia larvae is over 60%.

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PUM

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Abstract

The present invention relates to a culture method of river crab, in particular, it discloses an isolate embryo freeze-preservation method of mitten crab, it includes the following steps: stripping embryo from abdominal region of brooding mittn crab, placing the embryo in a low-temp. resistant container, storing said container in refrigerator, when it is used, taking out said container from refrigerator, placcing it in liquor with 20 deg.C and thawing, taking out the thawed embryo from thawed liquor, placing it into hatching apparatus to culture larva. Its low-temp. preservation temp. is 0- -200 deg.C.

Description

1. Technical field: [0001] The invention relates to a method for cultivating river crabs, and in particular discloses a method for cryopreserving isolated embryos of Chinese mitten crabs. 2. Background technology: [0002] Eriocheir sinensis, also known as hairy crabs, river crabs, and crabs, is produced in the three mouths of the Yangtze River in China. It belongs to China's special aquatic products. Due to the pollution of the ecological environment and people's overfishing, natural crab seedlings are on the verge of extinction, and people begin artificial breeding of river crabs. , Seedlings are concentrated in spring and summer, and artificial seedlings are strictly limited by seasonal temperature, which has caused difficulties for off-season and intensive breeding of river crabs. 3. Contents of the invention: [0003] The technical problem to be solved by the present invention is that artificial seedlings can be propagated and raised at any time without being restrict...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 朱钧
Owner 上海金蟹水产科技有限公司