Method for preparing biology diesel oil by using amylum enzymolysis to cultivate heterotrophy alga

A technology of biodiesel and heterotrophic algae, which is applied in the preparation of biological raw materials, liquid hydrocarbon mixtures, petroleum industry, etc., can solve the problems of rapid pyrolysis of heterotrophic algae to prepare biomass energy, etc., and achieve good application and market. Demand, increased yield, effect of high oil production

Inactive Publication Date: 2003-10-08
TSINGHUA UNIV
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Problems solved by technology

But so far, there is no literature on the rapid pyrolysis of heterotrophic algae to prepare biomass energy, and there is no literature on the preparation of culture medium for culturing heterotrophic algae by enzymatically hydrolyzing starch to make glucose aqueous solution, and then using heterotrophic algae to rapidly pyrolyze to prepare biomass liquid fuel.

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  • Method for preparing biology diesel oil by using amylum enzymolysis to cultivate heterotrophy alga
  • Method for preparing biology diesel oil by using amylum enzymolysis to cultivate heterotrophy alga
  • Method for preparing biology diesel oil by using amylum enzymolysis to cultivate heterotrophy alga

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Embodiment Construction

[0010] The present invention is a method for producing biodiesel by enzymatically hydrolyzing and cultivating heterotrophic algae for fast pyrolysis. Firstly, a culture solution is prepared by enzymatically hydrolyzing starch to prepare an aqueous solution of glucose, and the heterotrophic algae with a higher fat content than autotrophic Chlorella is obtained through heterotrophic transformation culture technology. Chlorella is raised, and heterotrophic algae cells with high fat content are quickly pyrolyzed in a fluidized bed. After gas and solid separation, the gas is condensed into liquid, which is biodiesel. Its preparation method is:

[0011] 1. Enzymolysis of starch to prepare heterotrophic chlorella culture solution: use α-amylase and glucoamylase to synergistically hydrolyze starch (starch raw materials can be corn flour, flour, wheat flour, etc.), or use Aspergillus niger extract or soil extract as a catalyst for hydrolysis Starch, determination of glucose content in ...

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Abstract

A process for preparing biologic diesel oil from starch includes enzymolyzing low-grade starch to obtain aqueous solution of glucose, preparing culture madium, heterotrophic transfer to obtain heterotrophic chlorella, and fust pyrolysis to obtain biologic diesel oil. Its advantages are high output rate, quality and heat value, and high flowability.

Description

technical field [0001] The invention belongs to the fields of bioengineering and energy, and relates to a method for rapidly pyrolyzing heterotrophic algae with high fat content to prepare biodiesel by enzymolyzing starch. Background technique [0002] With the decrease of fossil energy such as oil and coal and the air pollution caused by the combustion of fossil energy, people began to look for new energy, among which the most concerned is biomass energy. Biomass energy is a kind of renewable energy. After this part of energy is consumed, more biomass will be formed every year, so this energy will be inexhaustible and inexhaustible. Western developed countries, especially some developed countries in Europe, have taken advantage of their abundant biomass resources, including wood and crop straw, to research and develop a set of renewable bioenergy production technologies that use biomass pyrolysis to produce hydrocarbons. For example, the utilization of various types of ren...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C10G3/00
CPCY02P30/20
Inventor 吴庆余缪晓玲徐翰
Owner TSINGHUA UNIV
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