Technical method for extracting salvianolic acid B from radix salivae miltiorrhizae

A process method and technology of salvianolic acid, applied in the field of extraction of salvianolic acid B, can solve problems such as the difficulty of salvianolic acid B, and achieve the effect of large loading capacity of silica gel

Inactive Publication Date: 2004-09-15
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Due to the commonality of its structure, it is very difficult to separate high-purity salvianolic acid B. So far, there is no report to obtain its pure product. The highest content of salvianolic acid B in existing literature reports is only about 70%. Some are around 60% or even lower

Method used

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  • Technical method for extracting salvianolic acid B from radix salivae miltiorrhizae
  • Technical method for extracting salvianolic acid B from radix salivae miltiorrhizae
  • Technical method for extracting salvianolic acid B from radix salivae miltiorrhizae

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Experimental program
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Effect test

Embodiment 1

[0028] A product with a salvianolic acid B content of 80%

[0029] Extract 200 grams of salvia miltiorrhiza raw material with 6 times the amount of water for 2 hours, then extract with 4 times the amount of water for 1.5 hours, t=65 degrees Celsius; combine the two extracts, add 2% chitosan 100ml to flocculate for 1 hour, t=0 Celsius; filter, concentrate the filtrate under reduced pressure to twice the amount of the crude drug, add 1200ml of absolute ethanol to settle for 2 hours, t=0°C; filter, concentrate to remove ethanol, dilute to 2 times the amount of the crude drug with water, and use an equal volume Petroleum ether was extracted twice, the pH of the water phase was adjusted to 2, extracted twice with ethyl acetate, and the ethyl acetate phase was evaporated to dryness to obtain the total phenolic acid components, light yellow, the HPLC test results are shown in Figure 1, retention time 8.697min, it is salvianolic acid B, the content is 80%.

Embodiment 2

[0031] A product with a salvianolic acid B content of 90%

[0032] Mix the light yellow powder with salvianolic acid B content of 80% into an appropriate amount of silica gel, put it into the column, and elute with 1000ml of ethyl acetate:petroleum ether (1:1) mixed solution, discard the eluent, and then use 400ml Eluted with ethanol, and concentrated the eluate to dryness to obtain off-white powder, the HPLC test results are shown in Figure 2, the retention time is 8.272min, it is salvianolic acid B, and the content is 0%.

Embodiment 3

[0034] A product with a salvianolic acid B content of 96%

[0035] Mix the light yellow powder with salvianolic acid B content of 80% into an appropriate amount of silica gel, put it into the column, and elute with 2000ml of ethyl acetate:petroleum ether (1:1) mixed solution, discard the eluent, and then use 400ml Elute with ethanol, and concentrate the eluate to dryness to obtain a dark green powder. The HPLC test results are shown in Figure 3. The retention time is 8.328 min. It is salvianolic acid B with a content of 96%.

[0036] HPLC,H E NMR, IR, MS analysis:

[0037] IR, H E NMR, MS detection and analysis are salvianolic acid B, and the product is subjected to HPLC detection, and the results are shown in accompanying drawings 4, 5, and 6.

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Abstract

The invention is a technical method to extract salvianolic acid B from tanshen, organically combining several methods to extract salvianolic acid B, to develop a new technique proper to separate and purify salvianolic acid B. It firstly extracts by water, then flocculates by chitosan, successively makes alcohol precipitation, then extracts by petroleum ether and acetic ester, respectively, and finally adopts silica gel column chromatography to obtain higher purity salvianolic acid B. It is applied to industrialized production.

Description

technical field [0001] The invention relates to a new process for extracting salvianolic acid B from salvia miltiorrhiza. Background technique [0002] Salvia miltiorrhiza was first listed in "Shen Nong's Materia Medica" and listed as top grade. It is one of the commonly used medicines in traditional Chinese medicine and has a long history of clinical application. According to modern pharmacological studies, salvianolic acid compounds have strong antioxidant effects. It can remove superoxide anions and hydroxyl radicals in the body, inhibit lipid peroxidation, and improve microcirculation; Salvia miltiorrhiza is mainly used in the treatment of cardiovascular and cerebrovascular diseases and various related diseases, and has a wide range of applications. [0003] Salvianolic acid B in the water-soluble components of Salvia miltiorrhiza is one of the main active components, with high content and strong efficacy. The separation and purification route of salvianolic acid B, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/84
Inventor 张立国倪力军高秀蛟
Owner EAST CHINA UNIV OF SCI & TECH
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