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Novel anti-biological cell free redical oxidation injury mixture

A technology of oxidative damage and free radicals, applied in drug combinations, antineoplastic drugs, cardiovascular system diseases, etc., can solve the problems of damaged cell structure, poor protection effect and low content of water-soluble components in cells

Inactive Publication Date: 2005-01-12
谭敦宁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another classic antioxidant vitamin E, the English spelling of vitamin E is (α-Tocopherol or Vitamin E), many animal and plant foods also contain different amounts of vitamin E, vitamin E was discovered by American scientist Herbert M Evans in 1922 It was found that vitamin E is also widely used as an antioxidant. Vitamin E is a fat-soluble substance, distributed in the cell membrane, and its content in the cytoplasm is low.
Both vitamin C and vitamin E have an obvious defect as antioxidants, that is, under certain conditions, such as free iron ions in cells, vitamin C can also cause peroxidation, which may further damage those cell structures that need to be protected

Method used

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Embodiment approach 1

[0011] A new type of anti-biological cell free radical oxidative damage mixture composed of vitamin C, vitamin E, and melatonin is used to protect human oxyhemoglobin A from oxidation experiments, vitamin C, vitamin E, melatonin, human oxyhemoglobin A and others All chemicals were purchased from Sigma Chemicals (Sigma Chemicals, St. Louis, MO, USA).

[0012] Experimental steps:

[0013]1. Human oxyhemoglobin A is dissolved in TRS-hydrochloric acid buffer, the molar concentration of the buffer is 20 millimoles, containing 0.1 millimoles of ethylenediaminetetraacetic acid, and the pH is 7.4 (pH 7.4). The concentration of human oxyhemoglobin A is 0.3 mg / ml, and then placed in a constant temperature water bath at 30°C for warm bathing. As time goes on, human oxyhemoglobin A will continue to self-oxidize, and the degree of self-oxidation can be measured by spectroscopic The photometer measures its optical density at 575 nm to judge. This experiment is divided into 8 groups; Group...

Embodiment approach 2

[0015] Experimental study of the protective effect of vitamin C, vitamin E and melatonin combination on beta-amyloid peptide-induced neuronal cell death.

[0016] 1. Cell culture and treatment: Mouse hippocampal neurons (HT22 cell line) were cultured in DMEM medium containing 10% calf serum, 2 millimoles of glutamine, and 100 units of linked enzyme per milliliter 0.2 mg of cephalosporin and 25 mmol of HEPEBS. The cells were planted in a 96-well culture plate with a cell density of 10,000 / cm2, and placed in a 5% carbon dioxide incubator (37°C) for 12 hours; There are 9 groups. Group 1 is the normal control group without adding any reagents. Groups 2 to 9 are the test groups. First, add 50 micromoles of vitamin C to group 3; add 50 micromoles of vitamin E to group 4; add 50 micromoles of vitamin E to group 5; 50 micromoles; group 6 added vitamin C and vitamin E 50 micromoles each; group 7 added vitamin C and melatonin 50 micromoles each; group 8 added vitamin E and melatonin 50...

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Abstract

A mixture for preventing and treating the free radical oxidizing damage of biologic cells, such as presenile dementia, Parkinson's disease, cerebrosclerosis, apoplexy, cardiovascular disease, tumor, etc is prepared from VC, VE and melatonin.

Description

Technical field: [0001] The invention is a novel anti-biological cell free radical oxidative damage mixture in the field of biotechnology. Background technique: [0002] With the development of modern medical science and technology, people have realized the relationship between free radical oxidative damage of cells and health. More and more evidence shows that many diseases are caused by the imbalance of oxidation and anti-oxidation in the body. When the body's antioxidant function is low, excessive free radicals will attack cells and cause oxidative damage to cells; when the oxidative damage to cells reaches a certain level, the human body will produce various diseases. The pathological changes and diseases related to free radical oxidative damage that have been confirmed so far include aging, neurodegenerative diseases (Alzheimer's disease, Parkinson's disease, cerebral sclerosis), stroke, cardiovascular diseases (angina pectoris, myocardial ischemia, coronary artery dis...

Claims

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Application Information

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IPC IPC(8): A61K31/4045A61P9/10A61P17/16A61P25/16A61P25/28A61P27/12A61P35/00
Inventor 谭敦宁谭敦宪
Owner 谭敦宁
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