Water hyacinth biological inhibitor and its preparing method
A bioinhibitor and water hyacinth technology, applied in botanical equipment and methods, biocides, animal repellants, etc., to achieve the effects of high content, strong activity, and easy production
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Embodiment 1
[0026] The preparation of embodiment 1 water hyacinth biological inhibitor
[0027] Take the lantana leaves collected in the suburbs of Guangzhou, dry them at 70°C to constant weight, grind them into fine powder to make dry powder (about less than 100 mesh), add 30 grams of dry powder to 1000 ml of water, and extract at 4°C overnight , centrifuged at 10000g for 15 minutes, and the supernatant was taken, which was lantana extract. Add 50 ml of glycerin and 10 ml of Tween-20 at a concentration of 1% by weight to 940 ml of the extract, stir and mix evenly, and adjust the pH to 5.6 with acetic acid to obtain a liquid water hyacinth biological inhibitor.
Embodiment 2
[0028] The preparation of embodiment 2 water hyacinth biological inhibitors
[0029] Take the lantana leaves collected in the suburbs of Guangzhou, dry them at 80°C to constant weight, grind them into fine powder to make dry powder (about less than 100 mesh), add 100 grams of dry powder to 1000 ml of water, and extract at 10°C overnight , centrifuged at 10000g for 15 minutes, and the supernatant was taken, which was lantana extract. Add 100 ml of glycerin and 50 ml of TritonX-100 at a concentration of 1% by weight to 850 ml of the extract, stir and mix evenly, and adjust the pH to 5.8 with acetic acid to obtain a liquid water hyacinth biological inhibitor.
Embodiment 3
[0030] Example 3 Effect Test of Water Hyacinth Biological Inhibitors on Water Hyacinth Growth and Leaf Senescence and Death
[0031] Water hyacinth [Eichhornia crassipes (Mart.) Solms. Family Pontederiaceae] was collected from the river in Qingpu County, Shanghai, and then grown and propagated in tanks in the artificial climate chamber of the Institute of Plant Physiology and Ecology, Chinese Academy of Sciences. Select water hyacinth plants with similar size and shape (about 50g fresh weight per water hyacinth, 5 unfolded leaves, root length 20cm, and water part about 20cm) to cultivate in a plastic box (34×27× 19cm), culture experiment in artificial climate chamber, temperature 25-30°C, light 1000-2500lux (measured by modelAplab ML 4420 photometer). During the experiment, the culture medium in the plastic box was kept constant by adding distilled water. Put 5 water hyacinths in each box, every 4 boxes is a group, and the dosage is 0.17L / m2 , sprayed with the inhibitor prepa...
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