Method for skin beauty-care, instrument and cream therefor
A beauty instrument and skin technology, applied in the field of skin beauty, can solve problems such as inhibition and inability to provide enough fibroblasts, and achieve the effect of simple use and small size
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Embodiment 1
[0042] Example 1: In vitro cell experiment methods and procedures
[0043] Skin fibroblasts (ATCC No CRL-1762) are grown in standard Dulbecco's modified Eagle medium (DMEM) (additional 200mM L-glutamine, 50ug / ml gentamicin and 10% calf serum). The growth conditions are 37°C, 5% CO 2 . Any operation of the cells is carried out in a sterile environment. When the cells are confluent on the 10cm plate, all the culture fluid is aspirated and the remaining culture fluid is washed with 5ml phosphate buffered saline (PBS). Then add 10ml PBS to cover the adherent cells. Adjust the special disc for LED cells to Figure 4 The output intensity, power and wave width on the control panel. Place the LED light-emitting side down on a 10cm flat board (if attached Figure 4 As shown in the upper right picture), turn on the power, turn on the switch, and start timing at the same time.
[0044] Exposure for a predetermined time (such Figure 4 , 8, 9), turn off the switch, immediately aspirate PBS, a...
Embodiment 2
[0046] Example 2: Home-use LED eye mask and OL-1 cosmetic cream skin-beautifying and wrinkle-removing test
[0047] 1. Test steps
[0048] 1. Recruit women aged 20-55 who have wrinkles or eye skin conditions (including eye bags, dark circles, etc.) who need to be improved, and divide them into groups according to age;
[0049] 2. Test one wavelength of eye mask in each group, and subdivide into groups for each wavelength group, and each group will receive LED light irradiation treatment with different frequency and different time and intensity combination;
[0050] 3. Each treatment is treated once a week, and a course of treatment is 12 weeks (the effect is usually only obvious after 4 weeks);
[0051] 4. Professional beauticians and therapists independently record the curative effect once a week;
[0052] 5. The beautician uses the camera we provide to record the changes of the eye wrinkles of each therapist (synchronized with the form record);
[0053] 6. After the test is over...
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