Eel grass root stem sterile culturing and engineering seedling fast reproducing method
A technology for sterile seedlings and bitter grass, which is applied in the fields of gardening methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of transplanted habitat pollution physiological ecological test, origin habitat destruction, unfavorable viewing, etc. Simple, low cost and easy to operate
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example 1
[0023] Example 1. In November, 2003, the source of Erythrina fragrans was collected from East Taihu Lake, Suzhou, and 50 enlarged rhizome sections were cut from stolons, soaked in soapy water for 2 hours, washed with tap water, and rinsed overnight under running water. In the ultra-clean workbench, use 0.1% mercuric chloride to sterilize thoroughly for a certain period of time, wash with sterile water, and inoculate in the culture medium ①. Cultured in tissue culture room. The average light intensity is 2000lx, the daily light is 10h, and the temperature is about 25°C. Within 2 weeks, 18 strains of infected bacteria and dead materials were removed, and the remaining 32 strains could grow. Transfer the healthy seedlings to the culture medium ② and cover with the culture medium ③, and continue to cultivate in the tissue culture room, and the Erythrina fragrans begins to sprout and proliferate. Afterwards, a single plant of Erythrina fructus was transferred for bud proliferatio...
example 2
[0024] Example 2. The above-mentioned cultivation material is cleaned and transplanted into a glass cylinder covered with sterilized sandstone, and cultivated in a greenhouse. Change the water every week and supplement the Hoagland nutrient solution to strengthen the seedlings. This batch of materials was used in the ecological restoration project of eutrophic water body in Taihu Estuary, with an area of 1,000m 2 .
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