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Eel grass root stem sterile culturing and engineering seedling fast reproducing method

A technology for sterile seedlings and bitter grass, which is applied in the fields of gardening methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of transplanted habitat pollution physiological ecological test, origin habitat destruction, unfavorable viewing, etc. Simple, low cost and easy to operate

Inactive Publication Date: 2005-06-08
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, a large number of salvage can easily lead to the destruction of the original habitat; on the other hand, the bitter grass in the natural habitat often contains various algae and bacteria, which is likely to cause pollution of the transplanted habitat and errors in related physiological and ecological experiments, which is also not conducive to indoor viewing
Of course, at present, there are seeds of bitter grass to sow to make up for the lack of provenance, but this requires a suitable aquatic environment and a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] Example 1. In November, 2003, the source of Erythrina fragrans was collected from East Taihu Lake, Suzhou, and 50 enlarged rhizome sections were cut from stolons, soaked in soapy water for 2 hours, washed with tap water, and rinsed overnight under running water. In the ultra-clean workbench, use 0.1% mercuric chloride to sterilize thoroughly for a certain period of time, wash with sterile water, and inoculate in the culture medium ①. Cultured in tissue culture room. The average light intensity is 2000lx, the daily light is 10h, and the temperature is about 25°C. Within 2 weeks, 18 strains of infected bacteria and dead materials were removed, and the remaining 32 strains could grow. Transfer the healthy seedlings to the culture medium ② and cover with the culture medium ③, and continue to cultivate in the tissue culture room, and the Erythrina fragrans begins to sprout and proliferate. Afterwards, a single plant of Erythrina fructus was transferred for bud proliferatio...

example 2

[0024] Example 2. The above-mentioned cultivation material is cleaned and transplanted into a glass cylinder covered with sterilized sandstone, and cultivated in a greenhouse. Change the water every week and supplement the Hoagland nutrient solution to strengthen the seedlings. This batch of materials was used in the ecological restoration project of eutrophic water body in Taihu Estuary, with an area of ​​1,000m 2 .

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PUM

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Abstract

A method for the aseptic culture of vallisneria used for restoring the aquatic ecosystem and providing the good to fish, shrimp and crab and the spawning site for them, and the fast reproduction of its engineering seedlings is disclosed, which features use of tissue culture and enlarged reproduction.

Description

1. Technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for rapidly propagating high-quality engineering seedlings of bitter grass. 2. Background technology [0002] Vallisneria natans (Vallisneria natans) is a submerged herbaceous plant belonging to the genus Vallisneria natans. It grows in waters such as rivers and lakes with high transparency, deep silt, and slow water flow. This species has strong water purification ability and high tolerance to water pollution. It is often used as a pioneer species to purify water quality in aquatic ecosystem restoration and reconstruction projects. This species also contains more nutrients, so it is also a common bait for fish, shrimp and crabs, and in the breeding pond, this species can provide the above-mentioned aquatic products with places for spawning, molting and refuge, so it can be widely used in fish, shrimp and crabs. farming. At the same time, this species is also a decoration...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 安树青周长芳蒋金辉孙志毅钱程
Owner NANJING UNIV
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