Vitis botanical extractive, its preparation method and its novel usage as an antibacterials synergist

A technology of plant extracts and antibacterial drugs, applied in the direction of antibacterial drugs, medical preparations containing active ingredients, pharmaceutical formulas, etc., to achieve the effect of enhancing bactericidal efficacy, eliminating bacterial resistance and high product yield

Inactive Publication Date: 2005-06-22
王秀英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the health care function of grapes has increasingly attracted the attention of scholars from all over the world in recent years, and extensive research has been carried out on its chemical composition, pharmacological effects, and development and application, but so far, there has been no research on the use of grape plant extracts as antibacterial agents The research and application of potent agents

Method used

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  • Vitis botanical extractive, its preparation method and its novel usage as an antibacterials synergist
  • Vitis botanical extractive, its preparation method and its novel usage as an antibacterials synergist
  • Vitis botanical extractive, its preparation method and its novel usage as an antibacterials synergist

Examples

Experimental program
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Effect test

Embodiment 1

[0051]A plant extract of the genus Vitis, which uses water extraction, ethanol extraction and glycerin extraction to separate and purify grape fruit, grape roots, vines and leaves, grape skins, and grape seeds to obtain an extract. After mixing the separately obtained extracts, the final concentration drying, spray drying and freeze drying are carried out to obtain powders containing grape stilbene, resveratrol, oleanolic acid, β-sitosterol, gallic acid and flavonoids状 extracts.

Embodiment 2

[0052] Example 2 Separation and extraction

[0053] Soak the raisins in distilled water for 30 minutes, and then decoct them at 100°C for 30 minutes. After filtering to remove the residue, the active substances are separated and purified by resin adsorption, and finally sterilized at 100°C for 10 minutes to obtain extract 1 After crushing the grape roots, vines and leaves, add ethanol, filter to remove the residue, and then separate and purify the active substances by resin adsorption, and finally filter and sterilize to obtain the extract 2; After soaking the grape skins in distilled water, add acidic ethanol and filter Remove the residue, and then separate and purify the active substance by silica gel column chromatography, and finally filter and sterilize to obtain the extract 3. After crushing the grape seeds, add glycerin to extract at 150℃, filter to remove the residue, and then separate and purify by resin adsorption The active substance is finally filtered and sterilized t...

Embodiment 3

[0054] Example 3 Determination of active ingredients

[0055] The content of resveratrol was determined by HPLC; the content of flavonoids, β-sitosterol, and gallic acid were determined by spectrophotometry, GC-MS and TLC.

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Abstract

The invention discloses a vitis botanical extractive, its preparation method and its novel usage as an antibacterials synergist, wherein the preparation consists of employing water extraction method, ethanol extraction method and glycerol extraction method to extract grape fruit, grape root, vine, leaf, peel and seeds, obtaining extract, mixing and concentration drying, spray-drying and freeze drying.

Description

Technical field: [0001] The present invention relates to a plant extract, in particular to an extract obtained from different parts of the fruit, bark, root, vine, leaf, and seed of the Vitis plant; at the same time, the present invention also relates to the preparation method of the extract and its function New uses of antibacterial drug synergists. Background technique: [0002] Since Fleiming discovered penicillin in 1929 and Waksman discovered streptomycin in 1940, many effective antibiotics have been found from the secondary metabolites of microorganisms, and many semi-synthetic antibiotics have been developed from this, which play an important role in the prevention and treatment of bacterial infections. Important role. Due to the long-term and large-scale use of antibiotics, the drug resistance of bacteria has increased year by year. Once effective antibiotics can no longer inhibit or kill bacteria, this has become one of the main reasons for the decline in the efficacy of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61P31/04
Inventor 王秀英高荣黄鹤程朱冰张华平
Owner 王秀英
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