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Prepn and use of high-purity momordica glycoside V

A mogroside, high-purity technology, applied in the fields of food science, steroids, organic chemistry, etc., can solve the problems of reducing production costs, high production costs, low yields, etc., to reduce production costs, improve purity, product quality good purity

Inactive Publication Date: 2005-12-14
广西中医学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the existing disadvantages of low purity, low yield or high production cost of mogroside V, the present invention provides a method for preparing high-purity mogroside V, which can not only improve the purity of mogroside V, but also Can reduce production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment one: (1). Get 5 kg of Luo Han Guo, pulverize, use 20% ethanol 5000ml to heat and reflux extract for 10h, filter the extract, add 60% ethanol to the dregs and add 60% ethanol for 4000ml to heat and reflux to extract for 6h, combine the extract and concentrate under reduced pressure to Paste, get A.

[0014] (2). Separate A with a D101 type macroporous resin column (about 15 cm in inner diameter, 5 kg of D101 type macroporous resin), first deionized water and 20% ethanol were eluted to colorless, and then 35% ethanol eluent Eluted and collected the eluate, concentrated under reduced pressure to extract, and B was obtained.

[0015](3). Separate B with a normal-phase silica gel column (about 10 cm in inner diameter, 4 kg of silica gel, dry packing, dry loading), and use chloroform-methanol-water (60:40:1) as the eluent to collect The eluate of the spot with Rf=0.23 (the stationary phase is a normal phase plate, and the developing solvent is n-butanol-acetic aci...

Embodiment 2

[0017] Embodiment two: (1). Get grosvenoria grosvenori 4kg, pulverize, use 30% ethanol 5000ml to heat and reflux extract for 16h, filter the extract, add 35% ethanol to the dregs and add 35% ethanol to heat and reflux to extract for 6h, combine the extract and concentrate under reduced pressure to Paste, get A.

[0018] (2). Separate A with a D101 type macroporous resin column (about 15 cm in inner diameter, 5 kg of D101 type macroporous resin), first deionized water and 25% ethanol were eluted to colorless, and then 40% ethanol eluent Eluted and collected the eluate, concentrated under reduced pressure to extract, and B was obtained.

[0019] (3). Separate B with a normal-phase silica gel column (about 10 cm in inner diameter, 4 kg of silica gel, dry packing, dry loading), and use chloroform-methanol-water (60:40:5) as the eluent to collect The eluate of the spot with Rf=0.23 (the stationary phase is a normal phase plate, and the developing solvent is n-butanol-acetic acid-w...

Embodiment 3

[0021] Embodiment three: (1). Get Luo Han Guo 5kg, pulverize, use 65% ethanol 5000ml to heat and reflux extract for 10h, filter the extract, add 55% ethanol to the dregs and add 5000ml of ethanol to heat and reflux for 5h, combine the extract and concentrate under reduced pressure to Paste, get A.

[0022] (2). Separate A with a D101 type macroporous resin column (about 15 cm in inner diameter, 5 kg of D101 type macroporous resin), first deionized water and 30% ethanol were eluted to colorless, and then 50% ethanol eluent Eluted and collected the eluate, concentrated under reduced pressure to extract, and B was obtained.

[0023] (3). Separate B with a normal-phase silica gel column (about 10 cm in inner diameter, 4 kg of silica gel, dry packing, dry loading), and use chloroform-methanol-water (60:40:3) as the eluent to collect The eluate of the spot with Rf=0.23 (the stationary phase is a normal phase plate, and the developing solvent is n-butanol-acetic acid-water=4:1:1) is...

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Abstract

The present invention relates to the preparation process of high-purity momordica glycoside V. The preparation process includes: crushing grosvenor momordica, heated ethanol reflux extracting, separating the extract in D101 macroporous resin, deionized water and ethanol eluting to no color and collecting the eluate, decompression concentrating to obtain paste, separating in positive phase silica gel column with chloroform, methanol and water as eluent and collecting the eluate, decompression concentrating, separating in negative phase silica gel C-18 column, detecting the eluate with HPLC, merging momordica glycoside V components with the same retention time and purity of 90-98 % and over 98 % separately, decompression concentrating to obtain momordica glycoside V component with purity of 90-98 % and momordica glycoside V component with purity over 98 %. The present invention has raised momordica glycoside V purity and low cost.

Description

technical field [0001] The invention relates to a method for preparing mogroside V, in particular to a method for preparing mogroside V with a purity greater than 98%. Background technique [0002] Luo Han Guo, (Momordica grosvenori swingle), belongs to the fruit of perennial herbaceous vines of the family Cucurbitaceae. Luo Han Guo in the region is the best. In the past, Luo Han Guo has been used as traditional Chinese medicine and dried fruit for a long time. Luo Han Guo has the effects of quenching thirst, reducing heat and heat, relieving cough and reducing phlegm, cooling blood and nourishing lungs, and lowering blood pressure. It can be used to treat tuberculosis, asthma, gastritis, pertussis, acute and chronic Tracheitis and acute and chronic tonsillitis and other diseases. In recent years, people have not only studied the strong sweet substance mogroside V in Luo Han Guo as a sweetener, but also paid attention to its pharmacology. [0003] Public literature report...

Claims

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Application Information

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IPC IPC(8): A23L27/30C07J9/00
Inventor 韦松莫善列思秀玲
Owner 广西中医学院
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