Complete artificial method for breeding Ciona and simple continuous bait scattering device

A glass sea squirt and breeding method technology, applied in the field of artificial breeding, can solve the problems of low survival rate, poor operability, high cost, etc., and achieve the effects of rapid growth, low cost, and saving manpower and time

Inactive Publication Date: 2006-02-08
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings of high cost, poor operability, and low survival rate in the previous breeding methods, the present invention solves the problems of hatching, attaching and , aquaculture water quality management, feed composition, bait feed

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  • Complete artificial method for breeding Ciona and simple continuous bait scattering device

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Embodiment Construction

[0018] Below by embodiment the present invention will be further described.

[0019] The first step is to use the method of artificial induction to promote the natural spawning and ovulation of wild Ciona to obtain fertilized eggs; or to obtain fertilized eggs with a fertilization rate of 100% through artificial insemination. In a biochemical incubator with a temperature of 23°C and constant humidity, the fertilized eggs hatched for 12-14 hours; the tadpole larvae were transferred to a plastic petri dish with a rough bottom, and the water level was kept at 5-10 cm. At this time, most of the tadpole larvae heads were attached At the bottom of the petri dish; the tail of the tadpole larvae disappears within 24 hours, and the metamorphosis process is gradually completed.

[0020] The specific method of artificially inducing natural spawning and ejaculation of wild Ciona is as follows: When the natural water temperature is 10-16°C, collect adult Ciona from the harbor and breed the...

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Abstract

The invention relates to an artificial breeding of the glass ascidiology and a relative simple continuous throwing-bait device. Wherein the artificial breeding method comprises: first, attaining the zygote by the artificial insemination and incubating the zygote with disinfected seawater to the young body of polliwog in the plastic culture dish; secondly, moving the young body in metamorphosis into smaller container for the first-stage cultivation while the seawater has been boiled for disinfection, then feeding the diatom; thirdly, when the young body grown to the 1 cm, moving it into larger aquarium with the natural seawater and using the continuous throwing-bait device to feed the artificial bait formed by spirulina powder, integument, shrimp and crab appetizer and the core of egg; when the length of individual reaches 4-7 cm, and the gonad has grown up gradually, attaining the zygote by the method in first step. So the artificial breeding of glass ascidiology in its whole life can be completed.

Description

technical field [0001] The invention relates to an artificial breeding method for filter-feeding experimental animals, in particular to a complete set of methods for obtaining fertilized eggs of Ciona, hatching, attaching and cultivating juvenile ascidians and preparing artificial feed for ascidians. Background technique [0002] Ciona is a lower chordate with a free-swimming larval stage resembling a small tadpole with dorsal nerve cord, brain primordia, and notochord. Ciona was once a popular model for the study of embryonic biology. As early as the 19th century, the study of developmental biology began. It played a certain role in the discovery of cytoplasmic determinants and was one of the first animals to start the study of cell lineage. In recent years, the whole genome sequence of Ciona has been tested and released, hundreds of thousands of EST data have been released, a large number of developmentally expressed genes have been cloned, and hundreds of gene expression ...

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Application Information

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IPC IPC(8): A01K67/02
Inventor 相建海刘利平蔡难儿李富花董波
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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