Method for atom force microscope inducing single molecule DNA positoning mutation

Abstract

The method to induce monocular DNA directed mutation by atomic force microscope in biotechnology field comprises steps shown follow: (1) preparing mica substrate modified by aminosilane; (2) labelling the end of DNA sample; (3) straightening and preparing the labelled sample; (4) inducing mutation; (5) collecting and separating the induced DNA segment; (6) amplifying monocell; (7) detecting amplification by agarose gel electrophoresis; (8) sequenching sample DNA, and screening the mutated sample. The advantages of this invention include: precise location, high resolution, high efficiency, and simple operation.

Description

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AI Technical Summary

Benefits of technology

The inventors developed an improved technique for modifying specific parts or regions within certain areas of DNA (called genes) that control important physiological processes such as growth, differentiation, developmental timing, etc., through manipulation applied mechanically over small sections called microsites. By doing this they could potentially create new ways to study these changes throughout their entire body.

Problems solved by technology

Technologies for studying how cells behave under different conditions have been developed during their study process called atomic force microscopic technique. However, current methods require manual laborious steps like adjustment of pressure applied to the material being studied, leading to errors caused by operator error. There is thus room for improvement through automating these processes without requiring human input.

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