Method for the detection and/or identification of bacteria present in a sample

Bacteria, sample technology, applied in the field of bacteria devices, detection and/or identification of bacteria present in liquid or solid samples

Inactive Publication Date: 2006-05-03
BIOMERIEUX SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Furthermore, the method as described in this paten

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  • Method for the detection and/or identification of bacteria present in a sample
  • Method for the detection and/or identification of bacteria present in a sample
  • Method for the detection and/or identification of bacteria present in a sample

Examples

Experimental program
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Embodiment 1

[0061] Example 1 - Detection of Staphylococcus aureus on liquid selective medium including indicator

[0062] In this example, the attached figure 1 The device shown in for the detection and / or identification of bacteria present in a sample detects Staphylococcus aureus. For this purpose, 225 ml of medium (2) (peptone water, bioMérieux, ref 42043) was introduced together with 25 ml of milk as Stomacher  the first container (1) of the bag, and inoculate the mixture with 3×10 4 Staphylococcus aureus cells. Control test results were obtained as described above using medium and milk, but without S. aureus inoculation. The upper part of the 25ml syringe was used as the second container (4) containing the first air volume (9) of 13ml. Use selection medium (5) (Qi-Staph  , bioMérieux) and introduce 3 ml of this selection medium into a second container (4). In this example, the detection system (6) is an indicator compound (potassium tellurite) that causes the presence of Sta...

Embodiment 2

[0064] Detection of Salmonella typhimurium on embodiment 2-cellulose column

[0065] The first container (1) is the same as described in Example 1, but can be any type of first container. In the present embodiment, inoculate 3 * 10 2 Salmonella typhimurium cells. The second container (4) and transfer unit (3) used in this embodiment are as attached figure 2shown in . Control experiments were performed as described in Example 1.

[0066] For the selection medium (5), 0.5 ml of BPW medium containing 20 μg of novobiocin was impregnated into a 2 cm long and 0.7 cm diameter cellulose column (10). The cellulose column (10) is placed inside the second container (4), but not in contact with the second opening (8) of the transfer member (3). In this embodiment, the second container (4) comprises exactly one opening, which corresponds to the second opening (8) of said conveying part (3), with the result that a closed chamber is formed except for the opening (8) . Deposited on to...

Embodiment 3

[0067] Example 3 - Detection of motile Salmonella typhimurium on semi-solid selection medium

[0068] The first container (1) is the same as described in Example 1, but can be any type of first container. In the present embodiment, inoculate 3 * 10 2 Salmonella typhimurium cells. The second container (4) and transfer unit (3) used in this embodiment are as attached image 3 shown in . Control experiments were performed as described in Example 1.

[0069] 3ml of semi-solid medium (API M-medium  , bioMérieux, ref50120) is placed inside the second container (4) and used as a selection medium (5) including 40 μg / ml novobiocin, the level of the selection medium (5) not reaching the level of the transfer part (3) and the first A second opening (8) between the two containers (5). as attached image 3 As shown in , the second opening (8) is separated from the detection system (6) by a cylinder (11), which encourages the migration of bacteria through the selection medium (5), w...

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Abstract

The invention relates to a method for the detection and/or identification of bacteria present in a liquid or solid sample, characterized in that: the sample containing said bacteria is placed in a liquid medium in a first container; The second container of the system; install the transmission part between the first and the second container; apply a temperature T1 to the inside of the second container, and then apply a temperature T2 to the inside of the second container; the temperature T1 is greater than the temperature T2, so that A defined volume of culture medium is transferred from a first container to a second container; the presence or absence of bacteria is determined and/or the bacteria are identified in a detection system.

Description

field of invention [0001] The present invention relates to a method for detecting and / or identifying bacteria present in a liquid or solid sample. The invention also relates to a device for detecting and / or identifying bacteria present in such samples. Background of the invention [0002] Identification of bacteria in food is essential to public health. For example, bacteria of the Salmonella genus, which can be found in many food products, are the cause of many diseases in humans (typhoid fever, food poisoning). Similarly, the isolation and identification of Listeria bacteria is a major responsibility in monitoring agricultural hygiene and medical bacteriology. Thus, among bacteria of the Listeria genus, Listeria monocytogenes, which is known to be pathogenic in humans, can cause listeriosis, which is sometimes seen in immunosuppressed individuals and young children. Fatal (25-30% of cases). Therefore, it is very important to provide reliable and rapid assays for the de...

Claims

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Application Information

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IPC IPC(8): C12Q1/04B01L3/00C12M1/28C12M1/34
CPCB01L3/502B01L2300/0636B01L2300/0663B01L2300/0832B01L2300/18B01L2400/0442C12Q1/04Y10S436/809
Inventor J·V·梅卡德尔巴迪亚B·科兰V·阿特拉舍
Owner BIOMERIEUX SA
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