Determination of inorganic phosphorus and its diagnostic reagent kit

A diagnostic kit and inorganic phosphorus technology, applied in the field of medical testing and determination, can solve problems such as affecting the use and promotion, and the difference in the accuracy of sensitivity test results.

Inactive Publication Date: 2006-05-31
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with different reagents and testing routes, the sensitivity of detection and the accuracy of detection results will be very different, which directly affects the use and promotion of this method.

Method used

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  • Determination of inorganic phosphorus and its diagnostic reagent kit
  • Determination of inorganic phosphorus and its diagnostic reagent kit
  • Determination of inorganic phosphorus and its diagnostic reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment one (single dose)

[0067] Prepare the diagnostic kit for inorganic phosphorus in serum according to the following components and dosage:

[0068] Glycine buffer 80mmol / l,

[0069] Pyruvate 3mmol / l,

[0070] Oxidized mPMS 0.5mmol / l,

[0071] INT (tetrazolium salt) 0.5mmol / l,

[0072] Pyruvate dehydrogenase 5000U / l,

[0073] Ethylene glycol 50% (accounting for the total volume of the reagent).

[0074] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 37°C, reaction time 10 minutes, test main wavelength 490-500nm, test secondary wavelength 600nm or more, the volume ratio of the sample to be tested is 1:25, The reaction direction is positive reaction (absorbance rises, the same below).

[0075] Add the serum sample and the prepared single dose, and the two are automatically mixed in the analyzer, and the rise of the absorbance at 490-500nm is detected and recorded. A total of 31 reading points were detected, and the data was recorded ...

Embodiment 2

[0076] Embodiment two (two doses)

[0077] Prepare the diagnostic kit for inorganic phosphorus in serum according to the following components and dosage:

[0078] Reagent I——

[0079] Imidazole ~ hydrochloric acid buffer 100mmol / l,

[0080] Pyruvate 7mmol / l,

[0081] NBT (tetrazolium salt) 2mmol / l,

[0082] Glycerol 50% (accounting for the total volume of reagent I);

[0083] Reagent II——

[0084] Imidazole ~ hydrochloric acid buffer 100mmol / l,

[0085] Oxidized mPMS 1mmol / l,

[0086] Pyruvate dehydrogenase 10000U / l,

[0087] Ethylene glycol 50% (accounting for the total volume of reagent II).

[0088] Set on the automatic biochemical analyzer (Hitachi-7080): temperature 30°C, reaction time 15 minutes, test main wavelength 546nm, test secondary wavelength 660nm or more, the ratio of the total volume of the tested sample to reagent I and reagent II is 1:25, the ratio of the amount of reagent I to reagent II is 4:1, and the reaction direction is positive reaction.

[0...

Embodiment 3

[0090] Embodiment three (three doses)

[0091] Prepare the diagnostic kit for inorganic phosphorus in serum according to the following components and dosage:

[0092] Reagent I——

[0093] Triethanolamine buffer 120mmol / l,

[0094] Pyruvate 10mmol / l,

[0095] Propylene glycol 20mmol / l;

[0096] Reagent II——

[0097] Triethanolamine buffer 120mmol / l,

[0098] WST1 (tetrazolium salt) 4mmol / l,

[0099] Propylene glycol 50% (accounting for the total volume of reagent II);

[0100] Reagent III——

[0101] Triethanolamine buffer 120mmol / l,

[0102] Oxidized mPMS 2mmol / l,

[0103] Pyruvate dehydrogenase 15000U / l,

[0104] Ethylene glycol 50% (accounting for the total volume of reagent III).

[0105]Set on the automatic biochemical analyzer (Hitachi-7080): temperature 25°C, reaction time 20 minutes, test main wavelength 470nm, test secondary wavelength 546nm or more, the total volume of the tested sample and reagent I, reagent II and reagent III The ratio is 1:25, the consum...

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Abstract

The invention is about the measuring method of Inorganic Phosphates and its diagnosis reagent box. Reacting pyruvate dehydrogenase with pyruvate under the activation of Inorganic Phosphates in the sample of plasma or serum and so on, transferring oxidized mPVS to reduced mPVS, and transferring the colorless tetrazole salinization to formazan dyer with color with reduced mPVS, testing the ascending range of dominant wave-length380ú¡800nmabsorbance before and after the reaction and finally measuring the content of Inorganic Phosphates . This method has high specificity and would not be contaminated by material of internal and exogenous sources, and the result is precise and accurate. Diving the diagnosis reagent box into double-dose or three-dose can reduces the cross interaction of each element, keeps the stability of the reagent and deposits chronically. Using this method can realize the fast testing in common ultraviolet/ visible light analyzer or semiautomatic/automatic analyzer and doesní»t require special or additional apparatus, so the cost is low. Thus, this method can be easily promoted and applied in the whole industry.

Description

technical field [0001] The invention relates to a method for measuring inorganic phosphorus and a diagnostic kit prepared by using the method, belonging to the technical field of medical examination and measurement. Background technique [0002] About 87% of the phosphorus in the human body exists in the bones, and the phosphorus in the blood maintains a dynamic balance relationship with the phosphorus in the bones. On the other hand, there is a certain relationship between the concentration of calcium and phosphorus in the blood, and the product of the two is kept within a certain range. In milligrams, the product of the content of calcium and phosphorus in about 100 ml of blood plasma is 35-40 . In normal people, when blood calcium increases, blood phosphorus decreases, and vice versa. When the above product is greater than 40, calcium and phosphorus are deposited in the bone tissue in the form of bone salt; when it is greater than 70, metastatic calcification may occur;...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/32
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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