Fixed single-layer DNA probe orientation controlling and real-time monitoring method and apparatus
A DNA probe, real-time monitoring technology, used in biological testing, phase influence characteristic measurement, material inspection products, etc., can solve the problem of immobilized monolayer DNA probe is not ideal, time-consuming, expensive reagents, photobleaching, damage Immobilized monolayer and other problems, to achieve the effect of low cost, low reagent cost and good stability
Inactive Publication Date: 2006-08-02
HUNAN UNIV
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In fluorescence spectroscopy, DNA probes need to be fluorescently labeled, which has disadvantages such as time-consuming, expensive reagents, and photobleaching; atomic force microscopy requires expensive special equipment, and i
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Abstract
The present invention relates to a method for regulating, controlling and real-time monitoring immobilized monolayer DNA probe orientation and its equipment. Said equipment includes the following several portions: gold film, surface plasma subresonance sensing system formed from light source, parallel light tubes, p polarizing disk, prism, grating spectrograph and computer and conductive glass flow cell. Said invention also provides the connection relationship of all the above-mentioned portions and the concrete working principle and concrete operation steps of said method for regulating, controlling and monitoring immobilized monolayer DNA probe orientation and its change.
Description
technical field [0001] The invention belongs to an optical method and device for regulating and monitoring the orientation of DNA probes, in particular to an orientation of immobilized monolayer DNA probes based on the combination of electric field and surface plasmon resonance (Surface Plasmon Resonance, SPR) sensing Method and device for regulation and real-time monitoring. Background technique [0002] In the fields of biosensors, biochips, drug delivery systems, and nanocomputers, the regulation of the molecular orientation of immobilized monolayers has an important impact on the performance of related devices. These immobilized monolayer molecules are usually composed of some linear molecules, such as 16-mercaptohexadecanoic acid, DNA, bipyridine, etc., whose orientation can be reversibly regulated by electric field, pH or mechanical force. Monolayer DNA probes immobilized on the surface of metals, silicon wafers and other substrates are the basic technology of various...
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Inventor 王柯敏羊小海王青
Owner HUNAN UNIV
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