Method of purifying albuterol and/or clenbuterol and immune affinity chromatographic column

A technology of albuterol and albuterol rats, which is applied to chemical instruments and methods, other chemical processes, material inspection products, etc., can solve the problems of excessive waste of organic solvents, cumbersome processing, and long time required to achieve simplification and purification of the processing process Good results, analysis of the effect of quality improvement

Inactive Publication Date: 2006-09-13
CHINA AGRI UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

The pretreatment of these methods utilizes liquid-liquid distribution and conventional SPE column purification and separation, all of which hav...

Method used

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  • Method of purifying albuterol and/or clenbuterol and immune affinity chromatographic column
  • Method of purifying albuterol and/or clenbuterol and immune affinity chromatographic column

Examples

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Embodiment 1

[0026] Embodiment 1, the preparation of the immunochromatographic column of purifying albuterol and / or clenbuterol

[0027] 1. Preparation of albuterol polyclonal antiserum

[0028] Synthesis of albuterol hapten: Dissolve albuterol completely in methanol, evaporate methanol to dryness on a rotary evaporator to obtain an oil, shake and dissolve in 20ml absolute ethanol, add succinic anhydride with the same molar number as salbutamol while shaking , shaking at room temperature for 3 hours to obtain a white suspension, which is the albuterol hapten.

[0029] Preparation of the immunogen: The salbutamol hapten was coupled with the protein carrier bovine serum albumin (BSA) or ovalbumin (OVA) by the mixed acid anhydride method to prepare the BSA-SAL or OVA-SAL conjugate, namely the immunogen.

[0030] Animal immunization: New Zealand white rabbits are used as immunized animals, and the immunization dose is 1mg·ml -1 BSA-SAL conjugate. New Zealand rabbits were bred for several w...

Embodiment 2

[0054] Example 2. Immunochromatography of the salbutamol monoclonal antibody secreted by the monoclonal hybridoma cell line A-2-1 CGMCC No.1607 that is coupled with the salbutamol rabbit polyclonal antibody or has cross-reactivity to both salbutamol and clenbuterol Preparation of the column kit and its purification effect on albuterol and / or clenbuterol

[0055] 1. Preparation of kits for purification of albuterol and / or clenbuterol

[0056] It is mainly composed of box body, immunochromatographic column (IAC column), salbutamol, clenbuterol standard solution, washing solution, eluent I, eluent II, preservation solution, and sponge bracket. Holes and grooves. In the groove of the sponge bracket, there are reagent bottles containing albuterol, clenbuterol standard solution, washing solution, eluent I, eluent II, and preservation solution, and the hole of the sponge bracket is equipped with an IAC column. Wherein the immunochromatographic column is prepared in Example 1 couple...

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Abstract

A method for purifying salbutamol and/or clenbuterol by the special immunoaffinity chromatographic column carrying immunoaffinity adsorbent is disclosed. Said adsorbent is composed of solid carrier and its coupled salbutamol polyclonal or monoclonal antibody. It features that the chromatography is used to measure the contents of salbutamol and clenbuterol, having high correctness.

Description

technical field [0001] The invention relates to a method for purifying albuterol and / or clenbuterol and a special immunoaffinity chromatographic column thereof. Background technique [0002] With the development of life sciences, people have become more and more interested in the substances and their changes in living organisms, and the analysis of biological samples has become a necessary means to explore and discover the mysteries of life. Due to the complex components of biological samples, the concentration of analytes is low, and most of the sample volumes are small, which puts forward higher requirements for the selectivity and sensitivity of the analytical method. Immunoaffinity chromatography (IAC, immunoaffinity chromatography) is an analytical method that combines an immune response with a chromatographic analysis method. Its high selectivity and high affinity undoubtedly simplify the analysis process. In the analysis of veterinary drug residues, the simplest and...

Claims

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Application Information

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IPC IPC(8): B01J20/281G01N33/53
Inventor 沈建忠何方洋王建平史为民吴聪明
Owner CHINA AGRI UNIV
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