Aliphatic acid synthase inhibitor and method for making same and uses
A technology of fatty acid synthase and inhibitor, applied in the field of fatty acid synthase inhibitor and its preparation and application, can solve the problems of limited application value and insufficient inhibitory activity, and achieve the effect of large application value and high inhibitory activity
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Embodiment 1
[0023] Example 1, green tea warm leaching extract was treated with 1M sulfuric acid at 100°C to prepare fatty acid synthase inhibitor
[0024]The pulverized green tea is added to 10 times the weight of 60% ethanol, soaked at 30° C. for 2 hours with stirring, and centrifuged to take the supernatant to obtain the green tea warm extract. Add an equal volume of 2M sulfuric acid to the green tea warm-leaching extract, mix the final concentration of sulfuric acid to 1M, treat at a constant temperature at 100°C, take 50 μl at regular intervals and put it into 950 μl of 50% ethanol (total green tea warm-leaching extract was diluted 40 times), and 3 μl was added to the fatty acid synthase activity assay system to measure the enzyme activity Ai. The control is the warm infusion extract of green tea diluted 40 times, and 3 μl is added to the fatty acid synthase activity assay system to measure the enzyme activity A0. Taking the activity determined by the control as 1.0, A i / A0 is the ...
Embodiment 2
[0025] Example 2, green tea warm leaching extract was treated with 1M sulfuric acid at 70°C to prepare fatty acid synthase inhibitor
[0026] Obtain green tea warm-leaching extract by the same method as Example 1, add equal volume of 2M sulfuric acid to green tea warm-leaching extract, treat at constant temperature at 70°C, take 50 μl at regular intervals and put it in 450 μl of 50% ethanol (Total green tea warm infusion extract was diluted 20 times), 5 μl was taken and added to fatty acid synthase activity assay system to measure the enzyme activity Ai. The control is the green tea warm infusion extract diluted 20 times, and 5 μl is added to the fatty acid synthase activity assay system to measure the enzyme activity A0. Taking the activity determined by the control as 1.0, Ai / A0 is the relative remaining activity of the enzyme under the action of the treatment solution. The measurement results showed that the inhibitory activity of the treatment solution increased with the ...
Embodiment 3
[0027] Example 3, green tea warm leaching extract was treated with 1M sulfuric acid at 40°C to prepare fatty acid synthase inhibitor
[0028] Obtain green tea warm-leaching extract with the same method as Example 1, add equal volume of 2M sulfuric acid to green tea warm-leaching extract, treat at a constant temperature at 40°C, take 50 μl at regular intervals and put it in 450 μl of 50% ethanol (Total green tea warm infusion extract was diluted 20 times), 10 μl was added to fatty acid synthase activity assay system, and the enzyme activity Ai was measured. The control is the green tea warm infusion extract diluted 20 times, and 10 μl is added to the fatty acid synthase activity assay system to measure the enzyme activity A0. Taking the activity determined by the control as 1.0, Ai / A0 is the relative remaining activity of the enzyme under the action of the treatment solution. The measurement results showed that the inhibitory activity of the treatment solution increased with t...
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