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Cremastra appendiculata(D.Don)Makino artificial seed preparation method

A technology for artificial seeds and production methods, which is applied to gardening methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of high cost, large workload of seedling refining and transplanting, and restricting large-scale production. Low natural reproduction rate, making up for the effect of long production cycle

Inactive Publication Date: 2006-10-11
NANJING UNIV
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Problems solved by technology

In recent years, although we have made great breakthroughs in the research of tissue culture and in vitro rapid propagation of rhododendron orchids, the production cycle of test-tube seedlings is long, the cost is high, the workload of hardening and transplanting is heavy, and it is inconvenient to store and transport. , thus limiting the large-scale production

Method used

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  • Cremastra appendiculata(D.Don)Makino artificial seed preparation method

Examples

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Embodiment Construction

[0023] 1. Isolation and cultivation of endophytic fungi of Rhododendron orchid and preparation of fungal extract

[0024] (1) Isolation and identification of endophytic fungi

[0025] Separation: Rinse the freshly collected wild rhododendron orchid pseudobulbs with running water for 6-12 hours and rinse them with sterile water, then cut them into 0.4-0.6cm 3 Little pieces left and right. Disinfect the surface with 75% ethanol for 5-10 seconds, rinse with sterile water for 3-5 times, and then use 0.1% HgCl 2 Disinfect the surface for 10-15 minutes, rinse with sterile water for 3-5 times, inoculate the cut piece in a PDA plate, and incubate at a constant temperature of 25±0.5°C. After the fungal hyphae grow from the cut piece, pick the tip mycelia for purification nourish.

[0026] Identification: 2 genera of endophytic fungi have been identified.

[0027] ① Chromosporium sp. On the wheat gluten medium, the mycelium of the colony is initially white, stained white, then yell...

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Abstract

The invention belongs to field of crop seed artificial production technology, and provides a key technology for producing rhododendron orchid which is a medical herb and in shortage. The invention in detail relates to the breeding of original bulb of ghododendron orchid and establishing of floating line of original bulb, preparing the medium material for embedding the artificial seed shell, artificial endosperm and germination medium material, storing the said seed and sowing.

Description

1. Technical field [0001] The invention belongs to the technical field of plant artificial seed production. 2. Background technology [0002] Rhododendron orchid [Cremastra appendiculata (D.Don.) Makino] is a wild rare medicinal and ornamental plant of the orchidaceae Rhododendron orchid, mainly distributed in East Asia, especially in China's Guizhou and Sichuan (Acta PhytotaxonomicaSinica.2003, 41 : 263-266). The pseudobulb is used as medicine, and the medicinal material is called Shiitake mushroom, which is pungent, sweet, cold in nature, and slightly poisonous. It has the effects of clearing heat and detoxifying, moistening lung and relieving cough, promoting blood circulation and relieving pain, reducing swelling and dissipating stagnation, etc. Internal use can resist liver cancer, breast cancer, uterine cancer, etc. (Journal of Traditional Chinese Medicine.1996, 16:243-246; Planta Medica.2004, 70:171-173), externally used to treat sores, snake bites, skin scalds or b...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04C12N5/10
Inventor 张明生吴树敬张大栋杨永华王红孙登云缪律
Owner NANJING UNIV
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