Method for detecting residue of furaltadone through mediated monoclonal antibody and application

A technology of monoclonal antibody and furaltadone, which is applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems of backward detection methods and inability to meet the needs of export, and achieve short detection time, low cost of use, and analysis The effect of simple process

Inactive Publication Date: 2007-02-21
YANGZHOU UNIV
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Problems solved by technology

However, the original detection method in our country is only for nitrofuran technical drugs, and the detection method is extremely backward, which can no longer meet the export demand.

Method used

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  • Method for detecting residue of furaltadone through mediated monoclonal antibody and application

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Experimental program
Comparison scheme
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Embodiment Construction

[0037] (1) Antigen synthesis

[0038] First, furaltadone is reduced: the amino group is substituted for the nitro group in furaltadone to prepare a reduced furaltadone solution.

[0039] Antigen synthesis was coupled using the diazotization method. Dissolve 23mg of potassium nitrite in 1ml of distilled water, and adjust the temperature to 0-5°C in an ice-water bath; place the reduced furaltadone solution in a 37°C water bath for 20 minutes, cool the supernatant to about 4°C, and adjust the pH to 1 -2, then slowly add potassium nitrite solution and keep stirring, let it stand for 30 minutes; dissolve 100mg of carrier protein in 4ml of phosphate buffer, cool to about 4°C, slowly add the diazotization drug and keep stirring , in order to keep the pH stable during the addition process, add 0.2mol / L NaOH at the same time, adjust the pH value to 7.4, and react overnight at 4°C; then dialyze the coupled product in PBS at 4°C for 72-96h, change the medium every 6h No small molecular...

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Abstract

A method for detecting furantadone by using single clone antibody as fixator includes using single clone antibody resisting furantadone as fixator to set up ELISA fast detection manner for detecting furantadone residue and utilizing said manner to prepare detection kit and detection test paper for providing fast means to detect out furantadone residue.

Description

Technical field: [0001] The invention belongs to the field of drug residue detection. Background technique: [0002] Nitrofuran drugs are synthetic broad-spectrum antibiotics, which have very good antibacterial effect and pharmacokinetic properties. In addition to being used as an antibiotic in veterinary medicine, it has also been used as a growth-promoting agent for pigs, poultry and aquatic products. additives are widely used. However, in the course of long-term laboratory research, it was found that nitrofuran drugs and their metabolites can cause cancer and gene mutations in experimental animals. (EU Directive 1442 / 95) Legislates to prohibit the use of four drugs, including Furaltadone, Nitrofurazone, Nitrofurantoin and Furazolidone, in the production and processing of food of animal origin. Subsequently, the United States, Canada, Australia, New Zealand and Japan all banned the use of nitrofuran drugs. In March 2002, my country also listed nitrofuran drugs as banned...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/547G01N33/52
Inventor 金文杰秦爱建蔡宝亮余兵王倩倩陶宏锦邰红霞刘岳龙
Owner YANGZHOU UNIV
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