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Method of fast screening streptomyces lydicus mutant capable of generating streptolydigin

A technology of Streptomyces lydides and Streptomyces lydides, applied in the field of industrial microorganisms, can solve the problems of heavy screening workload, limited screening work efficiency, affecting research and application, etc., and achieves the goal of increasing screening speed and improving work efficiency Effect

Inactive Publication Date: 2007-03-14
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2005, Cell magazine reported the mechanism of lidodinian streptavidin inhibiting bacterial RNA polymerase, but there is currently no relevant report in China
Foreign literature shows that the yield of lididicine is low, which affects further research and application
In order to increase the yield of antibiotics, directed mutagenesis and random mutagenesis are usually used. The workload of screening after mutagenesis is very large, which limits the efficiency of screening.

Method used

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  • Method of fast screening streptomyces lydicus mutant capable of generating streptolydigin
  • Method of fast screening streptomyces lydicus mutant capable of generating streptolydigin

Examples

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Effect test

Embodiment 1

[0035] A method for rapidly screening the Streptomyces lydidei mutant strain producing streptolycin, comprising the following steps:

[0036] (1) Preparation of culture medium:

[0037] ① Preparation of shake flask seed medium: Take 2.5 grams of glucose, 15 grams of soluble starch, 1 gram of yeast extract powder, 2 grams of peptone, 0.75 grams of dipotassium hydrogen phosphate, 0.25 grams of sodium chloride, 0.25 grams of magnesium sulfate, and add water to 500ml , mix well, dispense into Erlenmeyer flasks, 50ml per bottle, and sterilize at 121°C for 20min;

[0038] ② Preparation of shake flask fermentation medium: Take 2.5 grams of glucose, 20 grams of soluble starch, 1 gram of peptone, 0.5 grams of dipotassium hydrogen phosphate, 0.25 grams of sodium chloride, 0.25 grams of magnesium sulfate, add water to 500ml, mix well, and pack Put into Erlenmeyer flasks, 45ml per bottle, and sterilize at 121°C for 20min;

[0039] (2) Seed cultivation and fermentation:

[0040] ① Shake...

Embodiment 2

[0059] A method for rapidly screening the Streptomyces lydidei mutant strain producing streptolycin, comprising the following steps:

[0060] (1) Preparation of culture medium:

[0061] ① Preparation of shake flask seed medium: Take 3 grams of glucose, 15 grams of soluble starch, 0.5 grams of yeast extract powder, 1 gram of peptone, 0.5 grams of dipotassium hydrogen phosphate, 0.2 grams of sodium chloride, 0.2 grams of magnesium sulfate, and add water to 500ml , mix well, dispense into Erlenmeyer flasks, 50ml per bottle, and sterilize at 120°C for 30min;

[0062] ② Preparation of shake flask fermentation medium: Take 2 grams of glucose, 30 grams of soluble starch, 1 gram of peptone, 0.5 grams of dipotassium hydrogen phosphate, 0.25 grams of sodium chloride, 0.25 grams of magnesium sulfate, add water to 500ml, mix well, and pack Put into Erlenmeyer flasks, 45ml per bottle, and sterilize at 12°C for 30min;

[0063] (2) Seed cultivation and fermentation:

[0064] ① Shake flask...

Embodiment 3

[0075] A method for rapidly screening the Streptomyces lydidei mutant strain producing streptolycin, comprising the following steps:

[0076] (1) Preparation of culture medium:

[0077] ① Preparation of shake flask seed medium: Take 1 g of glucose, 20 g of soluble starch, 1.5 g of yeast extract powder, 3 g of peptone, 0.2 g of dipotassium hydrogen phosphate, 0.1 g of sodium chloride, 0.5 g of magnesium sulfate, and add water to 500 ml , mix well, dispense into Erlenmeyer flasks, 50ml per bottle, and sterilize at 120°C for 30min;

[0078] ② Preparation of shake flask fermentation medium: Take 5 grams of glucose, 10 grams of soluble starch, 0.5 grams of peptone, 0.1 grams of dipotassium hydrogen phosphate, 0.1 grams of sodium chloride, 0.5 grams of magnesium sulfate, add water to 500ml, mix well, and pack Put into Erlenmeyer flasks, 45ml per bottle, and sterilize at 12°C for 30min;

[0079] (2) Seed cultivation and fermentation:

[0080] ① Shake flask seed culture: Inoculate ...

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Abstract

The present invention discloses process of fast screening lydistreptomycete mutant for producing lydistreptin. The process includes the following steps: compounding culture medium; seed culture and fermentation; preparing sample; Fourier transform infrared spectral analysis; data processing; and main component analysis and artificial neural network analysis on the infrared data. The process can screen out lydistreptomycete mutant for producing lydistreptin fast and raise mutation efficiency.

Description

technical field [0001] The invention belongs to the field of industrial microorganisms. Background technique [0002] Streptomyces luteogriseus was isolated and screened from soil by our laboratory, and was preserved by the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC) on April 13, 2006, (preservation address: Zhongguancun, Haidian District, Beijing Beiyi 13, Institute of Microbiology, Chinese Academy of Sciences), the preservation number is CGMCC1692. Lyddie streptomycin produced by Streptomyces lydida CGMCCl692 has anti-tumor, anti-HIV protease and anti-G + bacterial activity. In 2005, Cell magazine reported the mechanism of streptolycin inhibiting bacterial RNA polymerase, but there is no relevant report in China. Foreign literature shows that the yield of lididicin is low, which affects further research and application. In order to increase the yield of antibiotics, directed mutagenesis and random mutage...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/465
Inventor 元英进于凤鸣吴晓健
Owner TIANJIN UNIV
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