Nano liposome of tea plant oil, and preparation method

A nano-liposome, tea tree oil technology, applied in the directions of liposome delivery, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of skin irritation, unreported, epithelial cell and fibroblast toxicity, etc. , to achieve the effect of good slow release and good skin targeting

Inactive Publication Date: 2007-05-09
上海微纳科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN1785258A discloses a kind of preparation method of tea tree oil liposome, but the research about tea tree oil nano liposome has not been reported at home and abroad
[0004] Some components of tea tree oil, such as eucalyptus oil and degradation products of monoterpenes, may cause allergic reactions, and when used in high concentrations, they may cause irritation to the skin and toxicity to epithelial cells and fibroblasts
These deficiencies make the application of tea tree oil limited to a certain extent

Method used

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  • Nano liposome of tea plant oil, and preparation method
  • Nano liposome of tea plant oil, and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 tea tree oil nano liposome preparation

[0024] Weigh the prescribed amount of lecithin, cholesterol, tea tree oil, vitamin E, Tween-80, sodium deoxycholate, etc., add an appropriate amount of absolute ethanol, and sonicate until the suspension is uniform to obtain a lipid suspension. The hydration medium is placed on a constant temperature magnetic stirrer to maintain a specified temperature and stirring speed, and the lipid suspension is slowly dropped into it to obtain a liposome initial suspension with an ethanol concentration of 10%. Place in a water-bath ultrasonic instrument and sonicate until the tea tree oil nano-liposome suspension is translucent to obtain the tea tree oil nano-liposome suspension.

Embodiment 2

[0025] Embodiment 2 tea tree oil nano liposome morphology observation

[0026] Morphological observation: Dilute an appropriate amount of the product with distilled water to a lower concentration, drop it on a copper grid and place it until the film is uniform, evaporate the excess liquid, and observe the product shape under a transmission electron microscope. The product is spherical or oval liposome particles, which are dispersed and independent from each other, as shown in Figure 1.

[0027] Particle size distribution measurement: take appropriate amount of tea tree oil nano liposomes, filter with 0.5 μm filter membrane and dilute to appropriate concentration with distilled water, measure the particle size and distribution of tea tree oil nano liposomes with laser particle size analyzer. The average particle size is 65.0nm, and the particle size distribution is ±30.3nm, see Figure 2.

Embodiment 3

[0028] Example 3 in vitro release

[0029] A Franz diffusion cell is used, the upper chamber is a diffusion chamber, and the lower chamber is a receiving chamber, and tea tree oil and tea tree oil nanoliposomes are used for transdermal experiments. The treated mouse skin is fixed in the middle of the diffusion cell, the inner side of the skin is in contact with the receiving solution, and tea tree oil or tea tree oil nano liposomes are applied on the mouse skin. The diffusion cell is kept at a constant temperature of 36.0±0.5°C, a stirrer is added into the diffusion cell, and the constant speed is stirred at 60rpm. Start timing after 15 minutes of adding and equilibrating, and take samples at different time points. Pour out all the solution when sampling, and add an equal amount of receiving solution at the same time. The UV value of tea tree oil at each time point is calibrated with the UV value of the blank skin receiving solution without smearing te...

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Abstract

A nano-liposome of tea tree oil with high slowly-releasing performance and target to dermal peptide is prepared from tea tree oil, phosphatide, stabilizer, surfactant and flexible auxiliary through proportionally adding them to organic solvent, ultrasonic stirring, dripping constant-temp hydrating medium to obtain primary suspension, and ultrasonic treating by water bath to become semi-transparent product.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations or cosmetics, and in particular relates to a tea tree oil nano liposome and a preparation method thereof. Background technique [0002] Tea tree oil (tea tree oil) is a volatile essential oil extracted from the branches and leaves of several plants of the Myrtaceae Melaleuca genus. It is the most powerful natural antibacterial agent found so far. Composed of complex mixtures, about 100 kinds of chemical components can be isolated. The antibacterial activity is mainly due to mycoten-4-ol, while other components also play synergistic and individual roles. It has broad-spectrum antibacterial, antifungal, antiviral and anti-inflammatory activities, and is widely used in the daily chemical industry and medical industry. [0003] There are many patent applications for medical applications of tea tree oil at home and abroad. U.S. Patent No. 6,413,555 discloses a rubbing agent for the treatme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K36/61A61P31/00A61P29/00
Inventor 徐云龙钱秀珍王伟杨欣欣
Owner 上海微纳科技有限公司
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