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Method of preventing nodavirus infection and therapeutic method
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A Nodamura virus, viral technology, applied in the direction of viruses, medical raw materials derived from viruses/phages, antiviral agents, etc.
Inactive Publication Date: 2007-05-09
JAPAN SCI & TECH CORP
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However, this VHSV is a virus that targets internal organs such as the kidney, and there is no report that ABV is also effective against viral neuronecrosis, a disease of the central nervous system caused by Nodamura virus.
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Embodiment 1
[0034] (Properties of Aquatic BiRNA Virus (FBV Strain))
[0035] The aquatic biRNA virus (FBV strain) used in this example belongs to the aquatic biRNA virus genus of the family BiRNAviridae, is a spherical virus with a diameter of about 60 nm without an envelope (envelope), and has double-stranded RNA as a nucleic acid . This FBV strain also belongs to the aquatic diRNAvirus genus of the family DiRNAviridae, and the rabbit antiserum produced by YTAV (yellowtail ascites virus), which is known as a disease-causing virus of fish, was completely neutralized. And, so classified as the same serotype as YTAV. In addition, this FBV strain is serologically different from IPNV (infectious pancreatic necrosis virus, infectious pancreatic necrosis virus) known as a causative virus of salmonids belonging to the family BiRNAviridae. Furthermore, this FBV strain proliferated favorably in RTG-2 cells, which are strained cells derived from fish.
Embodiment 2
[0037] (Safety test of aquatic double RNA virus (FBV strain) flatfish)
[0038] Aquatic bisRNA virus (FBV strain) is obtained by culturing RTG-2 cells in culture medium (Eagle's MEM, fetal bovine serum). The FBV strain thus obtained was stored at -80°C. This cryopreserved strain was effective even after 6 months or more.
[0039] The FBV strain that so cryopreserves is inoculated in the muscle (inoculation amount: 10 6.9 TCID 50 / bar, the number of test bars: 20). It was observed for 3 weeks at a water temperature of 20°C, and as a result, no dead fish or abnormal fish were found. In addition, the pathogenicity of this FBV strain was not found in flatfish juveniles (0.5 g).
Embodiment 3
[0041] (Safety test of aquatic double RNA virus (FBV strain) to grouper)
[0042] The aquatic bisRNA virus (FBV strain) is the same as in Example 2, and is obtained by culturing RTG-2 cells in a medium (Eagle's MEM, fetal bovine serum). The obtained FBV strains were stored frozen (-80°C). This cryopreserved strain was effective even after 6 months or more.
[0043] The FBV strain that so cryopreserves is inoculated in the muscle of the grouper (artificial culture) of average body weight (inoculation amount: 10 7.1 TCID 50 / bar, the number of test bars: 20). Observed at a water temperature of 25°C for 3 weeks, no dead fish or abnormal fish were found.
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Abstract
A preventive or therapeutic method for infection of fish with nodavirus or diseases, such as viral neural necrosis, attributed to nodavirus infection. Prevention of infection of fish with nodavirus, or prevention or treatment of diseases, such as viral neural necrosis, developed by nodavirus infection can be accomplished by conducting percutaneous inoculation of fish with aquabirna virus through injection, etc., or immersing fish in a liquid containing aquabirna virus, or giving a feed having aquabirna virus mixed therein to fish.
Description
technical field [0001] The present invention relates to a method for preventing and treating Nodavirus infection, especially viral neuronecrosis and the like. In more detail, the present invention relates to a method for preventing and treating viral neuronecrosis caused by infection of Nodamura virus, etc., using non-pathogenic aquatic biRNA virus (Aquabirnavirus). Background technique [0002] Currently, about 40 species of marine fish are farmed in Japan in order to protect and secure marine fish resources. In addition, several species of fish fry are produced as seed for culture. Techniques for seed production and culture of this fish are also being developed, but difficulties are often faced in the control of diseases caused by infections, especially viral infections. For example, biRNA virus (birnavirus) infection caused by YAV (yellowtail ascites virus) to yellowtail (yellowtail) etc., and VNN caused by Nodamura virus infection to grouper and flounder etc. have been...
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