Method for preparation of white spirit by fermentation of waste distiller's grains
A technology for liquor and glutinous rice, applied in the field of microbial fermentation and liquor brewing, can solve the problems of difficult storage, large environment, easy to be mildewed and deteriorated, etc., and achieve the effects of reducing the emission of glutinous rice, simple koji-making process and rich aroma components.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0028] Pick 1 erbium of TR12 engineering bacterium slant test tube spores preserved at a temperature of 0~4°C, inoculate in starch medium (corn starch 20g / L, agar powder 20g / L, potassium nitrate 2g / L, magnesium sulfate heptahydrate 1.2g / L L. Potassium dihydrogen phosphate 2.7g / L, pH=5.0, sterilized at 121°C for 20min, cooled on an eggplant bottle on an inclined plane), cultured at 75% humidity and 34°C for 4 days, and stored at 4°C. Add 40 mL of sterile water and 1 drop of Tuwen 80 to the above-mentioned eggplant bottle, and scrape the spores with a sterile inoculation spatula to make a spore suspension for later use.
[0029] In a 250ml conical flask, mix 9.0g of bran, 1.0g of corn flour, 7.5mL of tap water, 0.2g of ammonium sulfate, 0.02g of potassium dihydrogen phosphate, and 0.02g of magnesium sulfate, moisten the ingredients for 2 hours, and sterilize at 121°C for 20 minutes. , cooled to 40°C, insert 0.5mL / bottle of the above-mentioned spore suspension under aseptic opera...
Embodiment 2
[0034] The fortified bran koji described in Example 1 was mixed with a total of 135 g of the production Daqu at a ratio of 1:5, and 200 mL of sterile water and 27 mL of distiller's yeast (SZ-E8 Saccharomyces cerevisiae) were added until the yeast cell concentration was 7.5×10 5 cells / mL, after shaking well, place in a constant temperature incubator at 25°C for 3 days. Add 720 g of sterilized discarded grains to the compound culture, then add 300 mL of tap water, seal it with a safety film, and ferment at a constant temperature of 25°C for 40 days.
[0035]After the fermentation, 269mL of 14% v / v liquor was distilled, the utilization rate of total starch reached 58.6%, and the alcohol conversion rate of the utilized starch reached a high level of 70.8%. The analysis results of distilled lees liquor original wine are shown in Table 1. Although no ethyl butyrate and ethyl caproate were detected, the alcohol content and aroma components of the 40-day fermented wine were significan...
Embodiment 3
[0037] Mix the fortified bran koji described in Example 1 with the production Daqu at a ratio of 1:5 in a total of 135 g, add 173 mL of sterile water and 54 mL of distiller's mother (SZ-E8 Saccharomyces cerevisiae: LII-1-1 Aroma Yeast = 1:1) , and to a yeast cell concentration of 7.5×10 5 cells / mL, after shaking well, place in a constant temperature incubator at 25°C for 3 days. Add 720 g of sterilized discarded grains to the compound culture, then add 300 mL of tap water, seal it with a safety film, and ferment at a constant temperature of 25°C for 43 days.
[0038] After the fermentation, 170mL of 16% v / v liquor was distilled, the utilization rate of total starch reached 62.4%, and the alcohol conversion rate of the utilized starch reached 46.1%. The analysis results of distilled lees liquor components are shown in Table 2. Although no components such as ethyl butyrate and ethyl caproate were detected, compared with distilled lees liquor without aroma-producing yeast in Tab...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com