Design of artificial genes for use as controls in gene expression analysis systems

a gene expression and gene technology, applied in the field of gene expression analysis systems using artificial genes as controls, can solve the problems of inability to hybridize the source with those controls, and the microarray measurement often appears to be systematically biased

Inactive Publication Date: 2005-05-05
GE HEALTHCARE SV CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0008] Accordingly, this invention provides a process of producing controls that are useful in gene expression analysis systems de...

Problems solved by technology

Microarray measurements often appear to be systematically biased, however, and the factors that contribute to this bias are many and ill-defined (Bowtell, D. L., Nature Genetics 21, 25-32 (1999); Brown, P. P....

Method used

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  • Design of artificial genes for use as controls in gene expression analysis systems
  • Design of artificial genes for use as controls in gene expression analysis systems
  • Design of artificial genes for use as controls in gene expression analysis systems

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Embodiment Construction

[0050] The present invention teaches Controls for use in gene expression analysis systems such as microarrays. Many have expressed interest in being able to obtain suitable genes and spikes as controls for inclusion in their arrays.

[0051] An advantage of the Controls of this invention is that a single set can be used with assay systems designed for any species, as these Controls will not be present unless intentionally added. This contrasts with the concept of using genes from “distantly related species.” For example, an analysis system directed at detecting human gene expression might employ a Bacillus subtilis gene as control, which may not be present in a human genetic material. But this control might be present in bacterial genetic material (or at least, cross hybridize), thus it may not be a good control for an experiment on bacterial gene expression. The novel Controls presented here provide an advantage over the state of the art in that the same set of controls can be used w...

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Abstract

Method of producing controls for use in gene expression analysis systems such as macroarrays, real-time PCR, northern blots, SAGE and microarrays. The controls are generated either from near-random sequence of DNA, or from inter- or intragenic regions of a genome. Ten specific control sequences are also disclosed. Also presented are methods of using these controls, including as negative controls, positive controls, and as calibrators of a gene expression analysis system.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application claims priority to U.S. provisional patent application Ser. Nos. 60 / 289,202, filed May 7, 2001; and 60 / 312,420, filed Aug. 15, 2001; the disclosures of which are incorporated herein by reference in their entireties.REFERENCE TO SEQUENCE LISTING SUBMITTED ON COMPACT DISC [0002] The present application includes a Sequence Listing filed on one CD-R disc, provided in duplicate, containing a single file named PB0120.ST25.txt, having 32 kilobytes, last modified on May 6, 2002, and recorded on May 6, 2002. The Sequence Listing contained in said file on said disc is incorporated herein by reference in its entirety. BACKGROUND OF THE INVENTION [0003] 1. Field of the Invention [0004] The present invention relates to a method of using artificial genes as controls in gene expression analysis systems. More particularly, the present invention relates to a method of producing Controls for use in gene expression analysis systems such a...

Claims

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Application Information

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IPC IPC(8): C12N15/09C07H21/02C07H21/04C12P19/34C12Q1/68
CPCC12Q1/6809C12Q2545/101
Inventor SAMARTZIDOU, HRISSITURNER, LEAHDANIEL, STEVENHOUTS, THOMAS
Owner GE HEALTHCARE SV CORP
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