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Novel immunogens and methods for discovery and screening thereof

a technology of immunogens and immunogens, applied in the field of immunogen identification, can solve the problems of not fully optimizing the extraction or identification of the full antigen repertoire, and the cost of production and delivery, so as to reduce or protect against pneumococcal colonization

Inactive Publication Date: 2013-02-14
CHILDRENS MEDICAL CENT CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides methods for identifying new immunogens that can be used as vaccines to protect against pneumococcal colonization and sepsis. These immunogens, such as SP0862, SP1534, and SP2070, elicit a strong cellular immune response and can be administered through mucosal immunization with an adjuvant to reduce nasal colonization. Overall, this approach offers a promising tool for developing effective vaccines against pneumococcal infections.

Problems solved by technology

Despite the effectiveness of the conjugate pneumococcal vaccines, problems with this approach remain including the expense of production and delivery, and resulting serotype replacement as demonstrated in several clinical trials and epidemiologic studies.
Additionally, current methods to identify immunogens focus on techniques that do not fully optimize the extraction or identification of the full antigen repertoire.

Method used

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  • Novel immunogens and methods for discovery and screening thereof
  • Novel immunogens and methods for discovery and screening thereof
  • Novel immunogens and methods for discovery and screening thereof

Examples

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example 1

Pneumococcal Immunogens

[0126]Protein Isolation

[0127]Initial efforts to isolate these proteins via gel filtration of the WCC supernatant did not yield discrete enough separation of proteins. Ultimately, preparative SDS gel transverse elution of the WCC supernatant improved separation of the proteins. WCC supernatant with SDS buffer was loaded into 10 wells of a precast 4%-12% Bis / Tris SDS gel with -100 μg protein / lane and run at 200V over 30 mM in MES-SDS buffer. This gel was equilibrated in 2 mM phosphate buffer for 20 mM in fresh buffer three times to minimize SDS. The equilibrated gel was cut to size to fit the BioRad Mini Gel Eluter apparatus. Proteins were transversely eluted through the thickness of the gel with 90 rnA of current for 20 mM. Eluates were collected into the elution chambers beneath the gel and harvested by vacuum apparatus. This method yielded fourteen eluates with one or two protein bands per eluate. The proteins within each eluate were visualized on silver stai...

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Abstract

The present application is generally directed to methods for identifying immunogens from organisms and pathogens, and in particular for identifying immunogens which when administered as vaccines elicit a cellular and / or humoral immune response. The present application is also directed to pneumococcal T-cell immunogens, and vaccine compositions comprising one or a combination of pneumococcal immunogens and methods for treating or preventing pneumococcal infections using the vaccines thereof. The present invention also encompasses use of the pneumococcal immunogens for diagnostic purposes to identify a subject with a pneumococcal infection.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit of priority under 35 U.S.C. §119(e) of U.S. Provisional Application No. 61 / 428,296 filed Dec. 30, 2010 and U.S. Provisional Application No. 61 / 428,305, also filed Dec. 30, 2010, and U.S. Provisional Application No. 61 / 313,450 filed Mar. 12, 2010 the contents of each of which are incorporated herein by reference in their entirety.FIELD OF THE INVENTION[0002]The present application is generally directed to methods for identifying immunogens from organisms and pathogens, and in particular for identifying immunogens which when administered as vaccines elicit a cellular and / or humoral immune response. The present application is also directed to pneumococcal T-cell immunogens, and methods and compositions thereof.BACKGROUND OF THE INVENTION[0003]Almost one million children in the developing world die of pneumococcal infections each year. Despite the effectiveness of the conjugate pneumococcal vaccines, proble...

Claims

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Application Information

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IPC IPC(8): A61K39/09A61P31/04G01N33/53A61K39/02C07K14/315
CPCA61K39/092C07K14/3156A61K2039/55544A61P11/00A61P31/00A61P31/04A61P31/10A61P31/12A61P33/00A61P37/02A61P37/04A61K2039/6087A61K2039/55583A61K2039/70C07K2319/00
Inventor MALLEY, RICHARDLU, YINGJIEMOFFITT, KRISTIN L.
Owner CHILDRENS MEDICAL CENT CORP
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