Uses for prevention or treatment of brain diseases using microrna
a brain disease and microrna technology, applied in the field of brain disease prevention or treatment, can solve the problems of loss of memory and intelligence, not being used to cure, and not being able to control the formation of amyloid beta, so as to prevent or treat the disease
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
example 2
of miR-485-3p Expression in Hippocampus and Cerebral Cortex of 5×FAD Mouse (RT-qPCR)
[0119](1) Research Methods
[0120]The 5×FAD transgenic mouse is an animal model of Alzheimer disease obtained by overexpressing mutant forms of APP and PSEN1, which exhibits severe accumulation of intraneuronal Aβ42 from about 6 weeks.
[0121]Given the results of Example 1, RT-qPCR was performed to confirm the expression of miR-485-3p in the dementia animal model. 5×FAD transgenic mice and wild-type (WT) mice were deeply anesthetized and sacrificed by decapitation. After excising the brain immediately, the hippocampus and cerebral cortex were dissected from the remaining brain structure. Total miRNA was isolated from the hippocampus using the PAXgene Tissue miRNA kit (Qiagen, USA) according to the manufacturer's instructions. cDNA was synthesized using the miScript II RT kit (Qiagen, USA), and qPCR was performed using the mmu_miR-485-3p miScript Primer Assay kit and the miScript SYBR Green PCR kit. The m...
example 3
n of Target Gene of miR-485-3p
[0124]In order to analyze the base sequence and target location of hsa-miR-485-3p, it was confirmed using a target prediction software (miRDB) that the 3′-untranslated region (UTR) of human-derived ELAVL2 is the target of hsa-miR-485-3p. It was confirmed that the identified seed sequence was conserved also in mmu-miR-485-3p and the 3′-untranslated region of mouse-derived ELAVL2.
[0125]FIG. 4 shows a list of the 3′-untranslated region (UTR) mRNAs of ELAVL2, and shows the target 3′-untranslated region (UTR) mRNAs of miR485-3p. The 5′ seed sequence of miR-485-3p (ELAVL2) is shown in blue color. Table 4 shows the base sequence and target location of mmu-miR485-3p. It was confirmed using a target prediction software (miRDB) that the 3′-untranslated region (UTR) of human-derived ELAVL2 is the target of mmu-miR-485-3p.
TABLE 4Analysis of base sequence and target location of mrnu-miR485-3pGeneSequence (5′ → 3′)mmu-miR-485-3pAGUCAUACACGGCUCUCCUCUCSEQ ID NOTargetTo...
example 4
ion of Expression of Amyloid Beta (Aβ) 42 and ELAVL2 in Hippocampus and Cerebral Cortex of 5×FAD Mouse
[0126](1) Research Methods
[0127]Given the results of Example 3, the expression of Aβ 42 and ELAVL2 in the hippocampus and the cerebral cortex of 5×FAD was investigated. After sacrificing an anesthetized mouse by decapitation, the brain was extracted immediately. After preparing a homogenate of the brain (hippocampus and cerebral cortex), western blot was conducted using anti-ELAVL2 antibody (Abcam, USA). The immunoreactive protein was visualized with a chemiluminescence reagent (GE Healthcare, UK) and was measured and quantified using a chemiluminescence analyzer (Fusion SL). Aβ 42 in the hippocampus and the cerebral cortex was quantified by using the mouse / rat amyloid beta (1-42) ELISA kit (IBL) according to the manufacturer's instructions.
[0128](2) Research Results
[0129]1) Confirmation of Aβ 42 Expression in Hippocampus and Cerebral Cortex
[0130]FIGS. 5A-5B show results of quantita...
PUM
| Property | Measurement | Unit |
|---|---|---|
| molecular weight | aaaaa | aaaaa |
| molecular weight | aaaaa | aaaaa |
| molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More