The invention discloses a juvenile gobiocypris rarus feed and a preparation method thereof. The juvenile gobiocypris rarus feed is prepared from fish meal, dextrin, a mineral substance mixture, soybean oil, a vitamin mixture, cellulose, spiral seaweed powder and a binding agent in a certain proportion. The preparation method comprises the following steps: 1, crushing raw materials; 2, respectively weighing the fish meal, the dextrin, the mineral substance mixture, the soybean oil, the vitamin mixture, the cellulose, the spiral seaweed powder and the binding agent; 3, uniformly the weighed fish meal, the dextrin and the cellulose; 4, mixing the mineral substance mixture, the vitamin mixture and the spiral seaweed powder, and continuously mixing; 5, adding the soybean oil for mixing; 6, adding the binding agent in water, mixing to prepare an emulsion, adding a mixture, and mixing; and 7, preparing the mixture into granules by using a granulating machine to obtain a granular feed. The juvenile gobiocypris rarus feed is reasonable in formula, and convenient to use; the survival rate of the juvenile gobiocypris rarus feed can reach above 98 percent, the requirement of daily growth of the juvenile gobiocypris rarus is met, the quantity of pathogenic microorganisms carried by live baits is reduced, and the quality uniformity of the juvenile gobiocypris rarus as an experimental animal is improved.