The utility model discloses a self-locking packing box which comprises a box body and a box cover, an opening is formed in at least one side of the box body, the box cover is used for sealing the opening, the box cover comprises a first cover plate, the first cover plate is connected with the first side of the opening, at least one first inserting tongue is arranged on the side, away from the first side, of the first cover plate, and at least one second inserting tongue is arranged on the side, away from the first side, of the first cover plate. A first inserting groove is formed in the joint of the first inserting tongue and the first cover plate; the inserting buckles are connected to the second side of the opening, the second side is opposite to the first side, the inserting buckles correspond to the first inserting grooves in a one-to-one mode, each inserting buckle comprises a buckle body capable of being folded relative to the box body and a second inserting tongue capable of being folded relative to the buckle body, and each second inserting tongue is provided with a guiding structure capable of being inserted into the corresponding first inserting groove; and the length of the second inserting tongue is 3mm-6mm longer than that of the first inserting groove. The self-locking packaging box realizes self-locking in a covering state, and the structure of the inserting buckle or the first inserting groove needs to be damaged if the self-locking packaging box needs to be opened, so that the self-locking packaging box has an anti-theft function.